Hyaluronidase 1/HYAL1 Antibody

Images

 
Immunohistochemistry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunofluorescence: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary ...read more
Immunofluorescence: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications Flow, IHC, PEP-ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Hyaluronidase 1/HYAL1 Antibody Summary

Immunogen
Peptide with sequence ENTRTKESCQAIKEY corresponding to internal region according to NP_009296.1, NP_695014.1, NP_695015.1, NP_695017.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_009296.1), isoform 2 (NP_695014.1), isoform 3 (NP_695015.1) and isoform 5 (NP_695017.1). Reported variants represent identical protein (NP_009296.1; NP_149349.2; NP_695013.1).
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HYAL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Peptide ELISA Detection limit 1:128000
  • Immunofluorescence
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human brain (cerebellum, frontal cortex) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 48.4 kDa band according to isoform 1 (NP_009296.1, NP_149349.2, NP_695013.1), 27.9 kDa band according to isoform 3 (NP_695015.1) and 19.4 kDa band according to isoform 5 (NP_695017.1). The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
Control
Hyaluronidase 1/HYAL1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 3
using
NBP1-00201 in the following applications:

Publications
Read Publication using NBP1-00201.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Hyaluronidase 1/HYAL1 Antibody

  • EC 3.2.1.35
  • FUS2
  • HYAL1
  • HYAL-1
  • Hyaluronidase 1
  • Hyaluronoglucosaminidase 1
  • hyaluronoglucosaminidase-1
  • LUCA1
  • luCa-1
  • LUCA1hyaluronidase-1
  • Lung carcinoma protein 1
  • MGC45987
  • NAT6
  • plasma hyaluronidase
  • tumor suppressor LUCA-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hyaluronidase 1/HYAL1 Antibody (NBP1-00201)(1)

Review for Hyaluronidase 1/HYAL1 Antibody (NBP1-00201) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-00201:
Filter by Applications
ELISA
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
ELISA Human 07/13/2009
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Summary

ApplicationELISA
Sample TestedHuman saliva
SpeciesHuman
CommentsThis antibody was raised against a HYAL1 peptide and it did not work with ELISA at various concentrations against the recombinat protein in a direct ELISA.

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: 1x PBS+0.05% Tween and 2% BSA, Primary Ab Incubation Time: 2hr, Incubation Temp:25C

Comments

CommentsThis antibody was raised against a HYAL1 peptide and it did not work with ELISA at various concentrations against the recombinat protein in a direct ELISA.

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FAQs for Hyaluronidase 1/HYAL1 Antibody (NBP1-00201) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Verified Customer
07/13/2009
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol HYAL1
Entrez