Hyaluronidase 1/HYAL1 Antibody

Images

 
Immunohistochemistry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunofluorescence: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary ...read more
Immunofluorescence: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications ELISA, Flow, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Hyaluronidase 1/HYAL1 Antibody Summary

Immunogen
Peptide with sequence ENTRTKESCQAIKEY corresponding to internal region according to NP_009296.1, NP_695014.1, NP_695015.1, NP_695017.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_009296.1), isoform 2 (NP_695014.1), isoform 3 (NP_695015.1) and isoform 5 (NP_695017.1). Reported variants represent identical protein (NP_009296.1; NP_149349.2; NP_695013.1).
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HYAL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human brain (cerebellum, frontal cortex) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 48.4 kDa band according to isoform 1 (NP_009296.1, NP_149349.2, NP_695013.1), 27.9 kDa band according to isoform 3 (NP_695015.1) and 19.4 kDa band according to isoform 5 (NP_695017.1). The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
Control
Hyaluronidase 1/HYAL1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 3
using
NBP1-00201 in the following applications:

Publications
Read Publication using NBP1-00201.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Hyaluronidase 1/HYAL1 Antibody

  • EC 3.2.1.35
  • FUS2
  • HYAL1
  • HYAL-1
  • Hyaluronidase 1
  • Hyaluronoglucosaminidase 1
  • hyaluronoglucosaminidase-1
  • LUCA1
  • luCa-1
  • LUCA1hyaluronidase-1
  • Lung carcinoma protein 1
  • MGC45987
  • NAT6
  • plasma hyaluronidase
  • tumor suppressor LUCA-1

Background

HYAL1 codes for a protein that may have a role in promoting tumor progression and blocking the TGFB1-enhanced cell growth, and is expressed highly in the liver, kidney, and heart, with weaker expression in the lung placenta, and skeletal muscle. There are seven isoforms of HYAL1, with the longest being isoform 1, which is only expressed in bladder and prostate cancer cells and is 435 amino acids long and weighs approximately 48 kDa. Current studies are being done on several diseases and disorders relating to this gene including lung carcinoma, lysosomal storage disease, diabetes mellitus, ovarian cancer, endometrial carcinoma, prostate cancer, and breast cancer. HYAL1 has also been shown to have interactions with GUSB, ARSB, IDUA, FAM107B, and COL2A1 in pathways such as the metabolic, lysosome, and Maroteaux-Lamy syndrome pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hyaluronidase 1/HYAL1 Antibody (NBP1-00201)(1)

Review for Hyaluronidase 1/HYAL1 Antibody (NBP1-00201) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-00201:
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ELISA
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
ELISA Human 07/13/2009
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Summary

ApplicationELISA
Sample TestedHuman saliva
SpeciesHuman

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Verified Customer
07/13/2009
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol HYAL1
Entrez