Human VAP-1 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human VAP-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Human VAP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VAP-1 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VAP-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VAP-1 showed linear curves that were parallel to the standard curves obtained ...using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VAP-1.
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• Specificity: Natural and recombinant human VAP-1
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: AOC3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human VAP-1 Quantikine ELISA Kit

• amine oxidase, copper containing 3 (vascular adhesion protein 1)
• AOC3
• Copper amine oxidase
• EC 1.4.3
• HPAO
• HPAOSSAO
• Semicarbazide-sensitive amine oxidase
• SSAO
• VAP1
• VAP-1
• VAP1EC 1.4.3.21
• VAP-1membrane primary amine oxidase
• Vascular adhesion protein 1

Background

VAP-1 is a type II transmembrane glycoprotein. It is expressed on endothelium, vascular smooth muscle, and adipocytes. The extracellular domain mediates lymphocyte rolling, adhesion, possibly extravasation during inflammation, and also possesses monoamine oxidase activity. Adipocytes can release a soluble form via metalloproteinase cleavage.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VAP-1/AOC3 (DVAP10)(4)

Publications using DVAP10

• Z Öksüz, E Üçbilek, MS Serin, S Yara?, GO Temel, O Sezgin Circulating vascular adhesion protein-1(VAP-1): a possible biomarker for liver fibrosis associated with chronic hepatitis B and C Braz. J. Microbiol., 2020-09-21;0(0):. 2020-09-21 [PMID: 32959205] (Human)
• FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk Systemic Inflammation Precedes Microalbuminuria in Diabetes Kidney Int Rep, 2019-06-21;4(10):1373-1386. 2019-06-21 [PMID: 31701047] (Human)
• E Bournazou, J Samuels, H Zhou, S Krasnokuts, J Patel, T Han, J Bencardino, L Rybak, SB Abramson, U Junker, KS Brown, M Attur Vascular Adhesion Protein-1 (VAP-1) as Predictor of Radiographic Severity in Symptomatic Knee Osteoarthritis in the New York University Cohort Int J Mol Sci, 2019-05-29;20(11):. 2019-05-29 [PMID: 31146362] (Human)
• M Kraemer, M Krawczyk, F Noor, F Grünhage, F Lammert, JG Schneider Increased Circulating VAP-1 Levels Are Associated with Liver Fibrosis in Chronic Hepatitis C Infection J Clin Med, 2019-01-17;8(1):. 2019-01-17 [PMID: 30658395] (Human)

FAQs for VAP-1/AOC3 (DVAP10). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AOC3