Human TRAIL/TNFSF10 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TRAIL/TNFSF10 Quantikine ELISA Kit Summary

• Background: The Quantikine Human TRAIL Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TRAIL in cell culture supernates, serum, plasma, cell lysates, and saliva. It contains NS0-expressed recombinant human TRAIL and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TRAIL showed linear curves that were parallel to the standard curve...s obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TRAIL
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• Specificity: Natural and recombinant human TRAIL
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TNFSF10

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TRAIL/TNFSF10 Quantikine ELISA Kit

• Apo-2 ligand
• APO2L
• Apo-2Ltumor necrosis factor (ligand) family, member 10
• APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
• CD253 antigen
• CD253
• Protein TRAIL
• TL2
• TNF-related apoptosis-inducing ligand
• TNFSF10
• TRAIL
• TRAILTNF-related apoptosis inducing ligand TRAIL
• tumor necrosis factor (ligand) superfamily, member 10
• tumor necrosis factor ligand superfamily member 10

Background

TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. Among TNF superfamily members, TRAIL is the most homologous to Fas Ligand, sharing 28% amino acid sequence identity in their extracellular domains. Human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TRAIL/TNFSF10 (DTRL00)(39)

Publications using DTRL00

• Tong, J;Alzahrani, B;Subbiah, SK;Rampal, S;Ramasamy, R;Ma, Z;Wu, X;Mok, PL; Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells Scientific reports 2025-07-03 [PMID: 40610482] (Human)
• Zappa, F;Muniozguren, NL;Conrad, JE;Acosta-Alvear, D; The integrated stress response engages a cell-autonomous, ligand-independent, DR5-driven apoptosis switch Cell death & disease 2025-02-15 [PMID: 39955274] (Human)
• Bate, KA;Genetzakis, E;Vescovi, J;Gray, MP;Celermajer, DS;McGuire, HM;Grieve, SM;Vernon, ST;Cartland, SP;Yang, JY;Kavurma, MM;Figtree, GA; Vascular Cytokines and Atherosclerosis: Differential Serum Levels of TRAIL, IL-18, and OPG in Obstructive Coronary Artery Disease Biomolecules 2024-09-04 [PMID: 39334884] (Human)
• N Kuehnle, SM Osborne, Z Liang, M Manzano, E Gottwein CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent, TRAIL-R1-mediated cell death Cell Death and Differentiation, 2023-02-18;0(0):. 2023-02-18 [PMID: 36801923] (Human)
• S Ravichandr, G Grubbs, J Tang, Y Lee, C Huang, H Golding, S Khurana Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2-infected individuals reveal unlinked immune signatures Science Advances, 2021-10-13;7(42):eabi6533. 2021-10-13 [PMID: 34644111] (Human)
• MC Kuo, WA Chang, LY Wu, YC Tsai, YL Hsu Hypoxia-Induced Epithelial-to-Mesenchymal Transition in Proximal Tubular Epithelial Cells through miR-545-3p-TNFSF10 Biomolecules, 2021-07-15;11(7):. 2021-07-15 [PMID: 34356656] (Human)
• E Hadad, S Rudnick-Gl, E Itzhaki, MY Avivi, I Grinberg, Y Elias, S Margel Engineering of Doxorubicin-Encapsulating and TRAIL-Conjugated Poly(RGD) Proteinoid Nanocapsules for Drug Delivery Applications Polymers, 2020-12-16;12(12):. 2020-12-16 [PMID: 33339090] (Human)
• YW Eom, R Akter, W Li, S Lee, S Hwang, J Kim, MY Cho M1 Macrophages Promote TRAIL Expression in Adipose Tissue-Derived Stem Cells, Which Suppresses Colitis-Associated Colon Cancer by Increasing Apoptosis of CD133+ Cancer Stem Cells and Decreasing M2 Macrophage Population Int J Mol Sci, 2020-05-29;21(11):. 2020-05-29 [PMID: 32485960] (Human)
• L Cheng, H Yu, JA Wrobel, G Li, P Liu, Z Hu, XN Xu, L Su Identification of pathogenic TRAIL-expressing innate immune cells during HIV-1 infection in humanized mice by scRNA-Seq JCI Insight, 2020-06-04;5(11):. 2020-06-04 [PMID: 32406872] (Humanized Mouse)
• A Masuda, Y Isobe, K Sugimoto, M Yoshimori, A Arai, N Komatsu Efficient recruitment of c-FLIPL to the death-inducing signaling complex leads to Fas resistance in natural killer-cell lymphoma Cancer Sci., 2020-01-31;111(3):807-816. 2020-01-31 [PMID: 31908105] (Human)
• EJ Schenck, KC Ma, DR Price, T Nicholson, C Oromendia, ER Gentzler, E Sanchez, RM Baron, LE Fredenburg, JW Huh, II Siempos, AM Choi Circulating cell death biomarker TRAIL is associated with increased organ dysfunction in sepsis JCI Insight, 2019-05-02;4(9):. 2019-05-02 [PMID: 31045578] (Human)
• F Rossignoli, C Spano, G Grisendi, EM Foppiani, G Golinelli, I Mastrolia, M Bestagno, O Candini, T Petrachi, A Recchia, F Miselli, G Rovesti, G Orsi, E Veronesi, G Medici, B Petocchi, M Pinelli, EM Horwitz, P Conte, M Dominici MSC-Delivered Soluble TRAIL and Paclitaxel as Novel Combinatory Treatment for Pancreatic Adenocarcinoma Theranostics, 2019-01-01;9(2):436-448. 2019-01-01 [PMID: 30809285] (Human)
• C Spano, G Grisendi, G Golinelli, F Rossignoli, M Prapa, M Bestagno, O Candini, T Petrachi, A Recchia, F Miselli, G Rovesti, G Orsi, A Maiorana, P Manni, E Veronesi, MS Piccinno, A Murgia, M Pinelli, EM Horwitz, S Cascinu, P Conte, M Dominici Soluble TRAIL Armed Human MSC As Gene Therapy For Pancreatic Cancer Sci Rep, 2019-02-11;9(1):1788. 2019-02-11 [PMID: 30742129] (Human)
• MV Arcidiacon, E Rimondi, E Maietti, E Melloni, V Tisato, S Gallo, JM Valdiviels, E Fernández, À Betriu, R Voltan, G Zauli, S Volpato, P Secchiero Relationship between low levels of circulating TRAIL and atheromatosis progression in patients with chronic kidney disease PLoS ONE, 2018-09-11;13(9):e0203716. 2018-09-11 [PMID: 30204795] (Human)
• V Tisato, S Gallo, E Melloni, C Celeghini, A Passaro, G Zauli, P Secchiero, C Bergamini, A Trentini, G Bonaccorsi, G Valacchi, G Zuliani, C Cervellati TRAIL and Ceruloplasmin Inverse Correlation as a Representative Crosstalk between Inflammation and Oxidative Stress Mediators Inflamm., 2018-07-26;2018(0):9629537. 2018-07-26 [PMID: 30147446] (Human)
• J Yang, FR LeBlanc, SA Dighe, CE Hamele, TL Olson, DJ Feith, TP Loughran TRAIL mediates and sustains constitutive NF-κB activation in LGL leukemia Blood, 2018-04-26;0(0):. 2018-04-26 [PMID: 29699990] (Human)
• D Morano, A Rolfo, V Tisato, A Farina, E Rimondi, G Scutiero, P Greco, G Bonaccorsi, T Todros Lower maternal serum tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) levels in early preeclampsia. A retrospective study Pregnancy Hypertens, 2018-02-07;12(0):1-5. 2018-02-07 [PMID: 29674187] (Human)
• J Wagner, CL Kline, L Zhou, V Khazak, WS El-Deiry Anti-tumor effects of ONC201 in combination with VEGF-inhibitors significantly impacts colorectal cancer growth and survival in vivo through complementary non-overlapping mechanisms J. Exp. Clin. Cancer Res., 2018-01-22;37(1):11. 2018-01-22 [PMID: 29357916] (Mouse)
• Roybal K, Williams J, Morsut L, Rupp L, Kolinko I, Choe J, Walker W, McNally K, Lim W Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Cell, 2016-09-29;167(2):419-432.e16. 2016-09-29 [PMID: 27693353] (Human)
• Expression of osteoprotegerin and its ligands, RANKL and TRAIL, in rheumatoid arthritis Sci Rep, 2016-07-12;6(0):29713. 2016-07-12 [PMID: 27403809] (Human)
• Clonal evolution in patients with chronic lymphocytic leukaemia developing resistance to BTK inhibition Nat Commun, 2016-05-20;7(0):11589. 2016-05-20 [PMID: 27199251] (Human)
• Mohr A, Yu R, Zwacka R TRAIL-receptor preferences in pancreatic cancer cells revisited: Both TRAIL-R1 and TRAIL-R2 have a licence to kill. BMC Cancer, 2015-07-03;15(0):494. 2015-07-03 [PMID: 26138346] (Human)
• Zhou C, Long Y, Yang H, Zhu C, Ma Q, Zhang Y TRAIL Is Decreased Before 20 Weeks Gestation in Women with Hypertensive Disorders of Pregnancy. PLoS ONE, 2015-06-01;10(6):e0128425. 2015-06-01 [PMID: 26030414] (Human)
• Choi S, Lee Y, Kwak P, Lee J, Kim S, Lee S, Phi J, Wang K, Song J, Song S, Joo K, Kim S Clinically applicable human adipose tissue-derived mesenchymal stem cells delivering therapeutic genes to brainstem gliomas. Cancer Gene Ther, 2015-05-29;22(6):302-11. 2015-05-29 [PMID: 26021486] (Human)
• Kang S, Kang D, Bakhtiar Ul Islam S, Je S, Kim J, Song J Silencing Daxx increases the anti-tumor activity of a TRAIL/shRNA Bcl-xL-expressing oncolytic adenovirus through enhanced viral replication and cellular arrest. Cell Signal, 2015-03-05;27(6):1214-24. 2015-03-05 [PMID: 25748050] (Human)
• Teng J, Hejazi S, Badr C, Tannous B Systemic anticancer neural stem cells in combination with a cardiac glycoside for glioblastoma therapy. Stem Cells, 2014-08-01;32(8):2021-32. 2014-08-01 [PMID: 24801379] (Human, Mouse)
• Ray A, Tian Z, Das D, Coffman R, Richardson P, Chauhan D, Anderson K A novel TLR-9 agonist C792 inhibits plasmacytoid dendritic cell-induced myeloma cell growth and enhance cytotoxicity of bortezomib. Leukemia, 2014-01-30;28(8):1716-24. 2014-01-30 [PMID: 24476765] (Human)
• Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function. J. Immunol., 2011-12-16;188(3):1002-10. 2011-12-16 [PMID: 22180616] (Human)
• Bem RA, Bos AP, Wosten-van Asperen RM, Bruijn M, Lutter R, Sprick MR, van Woensel JB Potential role of soluble TRAIL in epithelial injury in children with severe RSV infection. Am. J. Respir. Cell Mol. Biol., 2009-07-27;42(6):697-705. 2009-07-27 [PMID: 19635930] (Human)
• Niessner A, Hohensinner PJ, Rychli K, Neuhold S, Zorn G, Richter B, Hulsmann M, Berger R, Mortl D, Huber K, Wojta J, Pacher R Prognostic value of apoptosis markers in advanced heart failure patients. Eur. Heart J., 2009-02-04;30(7):789-96. 2009-02-04 [PMID: 19196721] (Human)
• Secchiero P, Lamberti G, Corallini F, Melloni E, Guarnotta C, Sebastiani A, Zauli G Conjunctival sac fluid contains elevated levels of soluble TRAIL: implications for the anti-tumoral surveillance of the anterior surface of the eye. J. Cell. Physiol., 2009-01-01;218(1):199-204. 2009-01-01 [PMID: 18780288] (Human)
• Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells. Stem Cells, 2008-09-04;26(11):2955-63. 2008-09-04 [PMID: 18772312] (Human)
• Chamoux E, Houde N, L'Eriger K, Roux S Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway. J. Cell. Physiol., 2008-08-01;216(2):536-42. 2008-08-01 [PMID: 18338379] (Human)
• Hoffmann O, Priller J, Prozorovski T, Schulze-Topphoff U, Baeva N, Lunemann JD, Aktas O, Mahrhofer C, Stricker S, Zipp F, Weber JR TRAIL limits excessive host immune responses in bacterial meningitis. J. Clin. Invest., 2007-07-01;117(7):2004-13. 2007-07-01 [PMID: 17571163] (Human)
• Corallini F, Celeghini C, Rizzardi C, Pandolfi A, Di Silvestre S, Vaccarezza M, Zauli G Insulin down-regulates TRAIL expression in vascular smooth muscle cells both in vivo and in vitro. J. Cell. Physiol., 2007-07-01;212(1):89-95. 2007-07-01 [PMID: 17352408] (Human)
• Masci P, Olencki T, Wood L, Rybicki L, Jacobs B, Williams B, Faber P, Bukowski R, Tong K, Borden EC Gene modulatory effects, pharmacokinetics, and clinical tolerance of interferon-alpha1b: a second member of the interferon-alpha family. Clin. Pharmacol. Ther., 2007-03-01;81(3):354-61. 2007-03-01 [PMID: 17339865] (Human)
• Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock. Am. J. Respir. Crit. Care Med., 2006-03-30;174(1):51-7. 2006-03-30 [PMID: 16574939] (Human)
• Gibellini D, Vitone F, Schiavone P, Re MC HIV-1 tat protein and cell proliferation and survival: a brief review. New Microbiol., 2005-04-01;28(2):95-109. 2005-04-01 [PMID: 16035254] (Human)
• Campioni D, Secchiero P, Corallini F, Melloni E, Capitani S, Lanza F, Zauli G Evidence for a role of TNF-related apoptosis-inducing ligand (TRAIL) in the anemia of myelodysplastic syndromes. Am. J. Pathol., 2005-02-01;166(2):557-63. 2005-02-01 [PMID: 15681838] (Human)

FAQs for TRAIL/TNFSF10 (DTRL00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFSF10