Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit Summary

• Background: The Quantikine Human Thrombomodulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Thrombomodulin in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human Thrombomodulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Thrombomodulin showed linear curves that ...were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Thrombomodulin.
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• Specificity: Natural and recombinant human Thrombomodulin. This kit also recognizes Thrombomodulin complexed with Thrombin, Protein C, and Thrombin/Protein C.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: THBD

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit

• BDCA3
• BDCA-3
• CD141 antigen
• CD141
• Fetomodulin
• THBD
• THRM
• Thrombomodulin
• TM

Background

Thrombomodulin (TM), also known as CD141 and BDCA3, is a transmembrane expressed on vascular endothelial cells (EC), arterial smooth muscle cells, monocytes, and macrophages. It binds Thrombin and enhances the Thrombin-mediated activation of the anti-coagulant Protein C and the anti-fibrinolytic TAFI/Carboxypeptidase B2. Thrombomodulin also inhibits the ability of Thrombin to activate several pro-coagulant proteins (e.g. Fibrinogen, Factor V, Factor XIII, PAR-1). Thrombomodulin exerts multiple anti-inflammatory effects and reduces the severity of disease involving chronic inflammation. In cancer, Thrombomodulin suppresses tumor angiogenesis, growth, and epithelial-mesenchymal transition. Soluble fragments of Thrombomodulin are elevated in the serum, urine, and synovial fluid during coagulation disorders, inflammation, and organ failure.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Thrombomodulin/BDCA-3 (DTHBD0)(13)

Publications using DTHBD0

• Serlo, M;Inkinen, N;Lakkisto, P;Valkonen, M;Pulkkinen, A;Selander, T;Pettilä, V;Vaara, ST; Fluid bolus increases plasma hyaluronan concentration compared to follow-up strategy without a bolus in oliguric intensive care unit patients Scientific reports 2024-09-06 [PMID: 39242877] (Human)
• M Brambilla, P Canzano, PD Valle, A Becchetti, M Conti, M Alberti, A Galotta, ML Biondi, PA Lonati, F Veglia, A Bonomi, N Cosentino, PL Meroni, GV Zuccotti, A D'Angelo, M Camera Head-to-head comparison of four COVID-19 vaccines on platelet activation, coagulation and inflammation. The TREASURE study Thrombosis Research, 2023-01-18;223(0):24-33. 2023-01-18 [PMID: 36702064] (Human)
• R Gualtierot, F Tafuri, R Rossio, M Rota, P Bucciarell, B Ferrari, A Giachi, C Suffritti, M Cugno, F Peyvandi, On Behalf Lung Ultrasound Findings and Endothelial Perturbation in a COVID-19 Low-Intensity Care Unit Journal of Clinical Medicine, 2022-09-15;11(18):. 2022-09-15 [PMID: 36143072] (Human)
• VE Fiestas So, NR da Costa F, CF Dos Santos, G Corrêa, MDC Cipitelli, M Dornelas R, LJ de Souza, PV Damasco, RV da Cunha, FB Dos Santos, LM de Oliveir, EL de Azeredo Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus Viruses, 2021-09-08;13(9):. 2021-09-08 [PMID: 34578370] (Human)
• SA El-Benhawy, NA Sadek, MM Kamel, AM Sharaf, IG Abderhman, MI Morsi, A Abobakr Study the relationship of endothelial damage / dysfunction due to occupational exposure to low dose ionizing radiation versus high dose exposure during radiotherapy Cancer Treat Res Commun, 2020-10-08;25(0):100215. 2020-10-08 [PMID: 33091734] (Human)
• SP Polster, A Sharma, C Tanes, AT Tang, P Mericko, Y Cao, J Carrión-Pe, R Girard, J Koskimäki, D Zhang, A Stadnik, SG Romanos, SB Lyne, R Shenkar, K Yan, C Lee, A Akers, L Morrison, M Robinson, A Zafar, K Bittinger, H Kim, JA Gilbert, ML Kahn, L Shen, IA Awad Permissive microbiome characterizes human subjects with a neurovascular disease cavernous angioma Nat Commun, 2020-05-27;11(1):2659. 2020-05-27 [PMID: 32461638] (Human)
• K Hoshino, Y Nakamura, T Nakano, A Watanabe, H Sheng, K Tachibana, H Ishikura Enhanced effect of recombinant human soluble thrombomodulin by ultrasound irradiation in acute liver failure Sci Rep, 2020-02-03;10(1):1742. 2020-02-03 [PMID: 32015385] (Mouse)
• L Garabet, CE Henriksson, ML Lozano, W Ghanima, J Bussel, E Brodin, MP Fernández-, C Martínez, R González-C, MC Mowinckel, PM Sandset Markers of endothelial cell activation and neutrophil extracellular traps are elevated in immune thrombocytopenia but are not enhanced by thrombopoietin receptor agonists Thromb. Res., 2019-11-30;185(0):119-124. 2019-11-30 [PMID: 31805421] (Human)
• FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk Systemic Inflammation Precedes Microalbuminuria in Diabetes Kidney Int Rep, 2019-06-21;4(10):1373-1386. 2019-06-21 [PMID: 31701047] (Human)
• S Kazune, A Caica, K Volceka, O Suba, U Rubins, A Grabovskis Relationship of mottling score, skin microcirculatory perfusion indices and biomarkers of endothelial dysfunction in patients with septic shock: an observational study Crit Care, 2019-09-11;23(1):311. 2019-09-11 [PMID: 31511042] (Human)
• KS Rana, C Pararasa, I Afzal, DA Nagel, EJ Hill, CJ Bailey, HR Griffiths, I Kyrou, HS Randeva, S Bellary, JE Brown Plasma irisin is elevated in type 2 diabetes and is associated with increased E-selectin levels Cardiovasc Diabetol, 2017-11-09;16(1):147. 2017-11-09 [PMID: 29121940] (Human)
• S Béland, P Vallin, O Désy, E Lévesque, SA De Serres Effects of Alloantibodies to Human Leukocyte Antigen on Endothelial Expression and Serum Levels of Thrombomodulin J. Thromb. Haemost, 2017-03-21;0(0):. 2017-03-21 [PMID: 28239987] (Human)
• Common genetic risk factors for venous thrombosis in the chinese population. Am J Hum Genet, 2013-01-17;92(2):177-87. 2013-01-17 [PMID: 23332921] (Human)

Bioinformatics

• Gene Symbol: THBD