Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit Summary

• Background: The Quantikine Human Sonic Hedgehog/Shh N-Terminus Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Shh-N in cell culture supernates, serum, and heparin plasma. It contains E. coli-expressed recombinant human Shh-N and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Shh-N showed linear curves that were paralle...l to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Shh-N.
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• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: SHH

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Sonic Hedgehog/Shh (DSHH00)(5)

Publications using DSHH00

• R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells Cells, 2020-08-20;9(9):. 2020-08-20 [PMID: 32825270] (Human)
• S Shao, T Qin, W Qian, X Li, W Li, L Han, D Zhang, Z Wang, Q Ma, Z Wu, E Wu, J Lei Cav-1 Ablation in Pancreatic Stellate Cells Promotes Pancreatic Cancer Growth through Nrf2-Induced shh Signaling Oxid Med Cell Longev, 2020-04-20;2020(0):1868764. 2020-04-20 [PMID: 32377291] (Human)
• I Giarretta, I Gatto, M Marcantoni, G Lupi, D Tonello, E Gaetani, D Pitocco, R Iezzi, A Truma, A Porfidia, A Visonà, P Tondi, R Pola Microparticles Carrying Sonic Hedgehog Are Increased in Humans with Peripheral Artery Disease Int J Mol Sci, 2018-12-09;19(12):. 2018-12-09 [PMID: 30544841] (Human)
• C Xu, C Zou, M Hussain, W Shi, Y Shao, Z Jiang, X Wu, M Lu, J Wu, Q Xie, Y Ke, F Long, L Tang, X Wu High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia Mucosal Immunol, 2018-06-04;0(0):. 2018-06-04 [PMID: 29867080] (Human)
• S Liu, Y Lv, XX Wan, ZJ Song, YP Liu, S Miao, GL Wang, GJ Liu Hedgehog signaling contributes to bone cancer pain by regulating sensory neuron excitability in rats Mol Pain, 2018-01-01;14(0):1744806918767. 2018-01-01 [PMID: 29607715] (Rat)

FAQs for Sonic Hedgehog/Shh (DSHH00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SHH