Human Serum Albumin Native Protein

SDS-Page: Albumin Native Protein [NBP1-97049] - SDS PAGE of Human Albumin. Lane 1: Non-Reduced Human Albumin. Lane 2: 5 uL Opal Prestained Marker. Lane 3: Reduced Human Albumin. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 63 kDa/Observed at 55 kDa; Reduced at 63 kDa. Non-reduced migrates farther on gel due to native albumin's capacity to bind ions, increasing its charge and, therefore, ability to migrate farther down gel.

Dot Blot: Albumin Native Protein [NBP1-97049] - Human Albumin Rhodamine Conjugated. Antigen: Rhodamine Conjugated Human Albumin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: None. Block for 1 HR at RT.. Using the Rhodamine format of this antibody.

Dot Blot: Albumin Native Protein [NBP1-97049] - Human Albumin Rhodamine Conjugated. Antigen: Rhodamine Conjugated Human Albumin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: None. Block for 1 HR at RT.. Using the Rhodamine format of this antibody.

SDS-Page: Albumin Native Protein [NBP1-97049] - SDS-PAGE results of Human Albumin. Lane 1: reduced human albumin. Lane 2: opal prestained molecular weight ladder. Lane 3: non-reduced human albumin. Load: 1ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.

• Reactivity: Hu
• Applications: DB, PAGE
• Concentration: LYOPH

Human Serum Albumin Native Protein Summary

• Description: Native Human Albumin
  
  Source: Normal serum (Accession #:P02768)
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Albumin and anti-Human Serum.
• Protein/Peptide Type: Native Protein
• Gene: ALB
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • SDS-Page
• Application Notes: This product has been tested in SDS-Page and can be used in SDS, Western Blotting, ELISA experiments and other immunological assays.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 500 ul of deionzied water.

Alternate Names for Human Serum Albumin Native Protein

• albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Human albumin or serum albumin is encoded by the ALB gene and is the most abundant plasma protein in mammals. Human albumin is essential for maintaining the osmotic pressure needed for proper distribution of body fluids between intravascular compartments and body tissues. Human albumin also acts as a plasma carrier by non-specifically binding several hydrophobic steroid hormones and as a transport protein for hemin and fatty acids. Too much serum albumin in the body can be harmful.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ALB
• Uniprot:
  • P02768(Human)