Human Serum Albumin Native Protein

Dot Blot of Albumin Native Protein Conjugated. Antigen: Rhodamine Conjugated Human Albumin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: None. Block for 1 H at RT.

Dot Blot of Albumin Native Protein Conjugated. Antigen: Rhodamine Conjugated Human Albumin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: None. Block for 1 H at RT.

• Reactivity: Hu
• Applications: WB, DB, IHC
• Conjugate: Rhodamine
• Concentration: LYOPH

Human Serum Albumin Native Protein Summary

• Description: Native Human Albumin
  
  Source: Chromatographically pure Human Albumin
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
• Preparation Method: This product was prepared from chromatographically pure Human Albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Albumin.
• Protein/Peptide Type: Native Protein
• Gene: ALB
• Purity: Chromatography

Applications/Dilutions

• Dilutions:
  • Dot Blot 7.4 ng to 200 ng
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:100-1:500
• Application Notes: This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Chromatography
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water.

Alternate Names for Human Serum Albumin Native Protein

• albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Human albumin or serum albumin is encoded by the ALB gene and is the most abundant plasma protein in mammals. Human albumin is essential for maintaining the osmotic pressure needed for proper distribution of body fluids between intravascular compartments and body tissues. Human albumin also acts as a plasma carrier by non-specifically binding several hydrophobic steroid hormones and as a transport protein for hemin and fatty acids. Too much serum albumin in the body can be harmful.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ALB