Human PSGL-1/CD162 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human PSGL-1/CD162 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human PSGL-1/CD162 DuoSet ELISA, 5 Plate

• CD162 antigen
• CD162
• P-selectin glycoprotein ligand 1
• PSGL1
• PSGL-1
• PSGL-1cutaneous lymphocyte-associated associated antigen
• selectin P ligandCLA
• SELPLG

Background

PSGL-1 (P-Selectin Glycoprotein Ligand 1) is type I transmembrane receptor found on the surface of neutrophils, monocytes, and most lymphocytes. It is expressed as a dimeric mucin-like glycoprotein that is N-glycosylated and contains both sialylated and fucosylated O-linked oligosaccharides. PSGL-1 binds to P-, E- and L-Selectins. The calcium-dependent high affinity interaction with P-, E-, and L-Selectin mediates the tethering and rolling of neutrophils, monocytes, and/or lymphocytes on endothelial cells.

Publications for PSGL-1/CD162 (DY3345-05)(3)

Publications using DY3345-05

• Scalia, A;Coquay, M;Kindt, N;Duez, P;Aro, R;Journé, F;Fabjanczyk, M;Trelcat, A;Carlier, S; In Vitro Modulation of Human Foam Cell Formation and Adhesion Molecules Expression by Ginger Extracts Points to Potential Cardiovascular Preventive Agents International journal of molecular sciences 2024-08-31 [PMID: 39273434] (Human)
• Reis-Goes, FS;Silva, NN;Gondim, TM;Figueiredo, RG;Evangelista, GAO;Marchioro, SB;Costa, RS;Torres, AJL;Meyer, RJ;Trindade, SC;Fortuna, V; Exploring dysregulated immune response genes and endothelial dysfunction biomarkers as predictors of severe COVID-19 International immunopharmacology 2023-07-05 [PMID: 37453154] (Human)
• A Mariscal, C Zamora, C Díaz-Torné, MÀ Ortiz, JJ Agustín, D Reina, P Estrada, P Moya, H Corominas, S Vidal Increase of Circulating Monocyte-Platelet Conjugates in Rheumatoid Arthritis Responders to IL-6 Blockage International Journal of Molecular Sciences, 2022-05-20;23(10):. 2022-05-20 [PMID: 35628558] (Human)

FAQs for PSGL-1/CD162 (DY3345-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.