Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA, 2 Plate

Lysates prepared from HepG2 human hepatocellular carcinoma cells were incubated in wells coated with Human/Mouse/Rat Total SOD2/Mn-SOD Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. The same lysate and captured protein were electrophoresed, transferred to a PVDF membrane, and immunoblotted with a Human/Mouse/Rat SOD2 Polyclonal Antibody (R&D Systems®, Catalog # AF3419). Only the band corresponding to SOD2 was detected in captured material. To further determine specificity, recombinant human SOD1 and recombinant human SOD3 were assayed at 200 ng/mL and did not cross-react or interfere in the assay.

Lysates prepared from HepG2 human hepatocellular carcinoma cells, A172 human glioblastoma cells, HeLa human cervical epithelial carcinoma cells, Jurkat human acute T cell leukemia cells, and MDA-MB-453 human breast cancer cells were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of human/mouse/rat SOD2 detected by Western Blot.

Human peripheral blood mononuclear cells (PBMCs) were cultured for 24 hours in the absence or presence of 1 mM lipopolysaccharide (LPS). The lysates were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of human/mouse/rat SOD2 detected by Western Blot. Amounts of SOD2 as quantified by the Human/Mouse/Rat Total SOD2/Mn-SOD DuoSet® IC ELISA are consistent with the relative amounts of SOD2 determined by qualitative Western blot analysis.

Lysates prepared from DA3 mouse myeloma cells, L-929 mouse fibroblast cells, and PC-12 rat adrenal pheochromocytoma cells were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of SOD2 detected by Western Blot.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Conjugate: Biotin

Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA, 2 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: SOD2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA, 2 Plate

• EC 1.15.1.1
• indophenoloxidase B
• IPOB
• IPO-B
• manganese-containing superoxide dismutase
• mangano-superoxide dismutase
• Mn SOD
• Mn superoxide dismutase
• MnSOD
• MVCD6
• SOD, Mitochodrial
• SOD2
• superoxide dismutase [Mn], mitochondrial
• superoxide dismutase 2, mitochondrial

Background

Superoxide Dismutases, originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Three mammalian isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SODs are typically soluble secreted or cytosolic proteins, but are also found in the mitochondria and extracellular matrix.

Any of three metals, manganese, iron, or copper, may be used in the active site of SOD and are indicative of cellular localization.MnSOD (SOD2) is found in the mitochondria of both prokaryotes and ukaryotes, whereas the cytosol of eukaryotes and prokaryotic organisms contains Cu/ZnSOD (SOD1) and FeSOD, respectively.

There have been several mutations identified in the Cu/ZnSOD (SOD1) gene in amyotrophic lateral sclerosis (ALS) patients, possibly suggesting a role for free radicals in this disease process. The mechanism of motor neuron degeneration that occurs in ALS, however, is unclear at this time. Several hypotheses have been explored for the role of mutant SOD1 and convincing evidence for the involvement of apoptosis has been presented, as well.

Publications for SOD2/Mn-SOD (DYC3419-2)(25)

Publications using DYC3419-2

• Peng, Z;Zhang, W;Hong, H;Liu, L; Effect of luteolin on oxidative stress and inflammation in the human osteoblast cell line hFOB1.19 in an inflammatory microenvironment BMC pharmacology & toxicology 2024-07-12 [PMID: 38997762] (Human)
• Sun, L;Fang, K;Yang, Z; Combination therapy with probiotics and anti-PD-L1 antibody synergistically ameliorates sepsis in mouse model Heliyon 2024-06-15 [PMID: 38828304] (Mouse)
• Kim, H;Jin, B;Lee, C;Kim, D;Lee, A;Lee, S;An, H; Anti-Inflammatory Effect of Chestnut Honey and Cabbage Mixtures Alleviates Gastric Mucosal Damage Nutrients 2024-01-29 [PMID: 38337674] (Rat)
• M Mohany, MM Ahmed, SS Al-Rejaie The Role of NF-kappaB and Bax/Bcl-2/Caspase-3 Signaling Pathways in the Protective Effects of Sacubitril/Valsartan (Entresto) against HFD/STZ-Induced Diabetic Kidney Disease Biomedicines, 2022-11-09;10(11):. 2022-11-09 [PMID: 36359384] (Rat)
• OM David, KL Lategan, MF de Cortale, EJ Pool The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO2NPs) Using Caco-2 Cells Biomolecules, 2022-09-21;12(10):. 2022-09-21 [PMID: 36291543] (Human)
• LQ Chen, WS Wang, SQ Li, JH Liu Minocycline relieves myocardial ischemia-reperfusion injury in rats by inhibiting inflammation, oxidative stress and apoptosis European review for medical and pharmacological sciences, 2022-04-01;26(8):3001-3009. 2022-04-01 [PMID: 35503600] (Rat)
• R D'Amico, E Gugliandol, M Cordaro, R Fusco, T Genovese, AF Peritore, R Crupi, L Interdonat, D Di Paola, S Cuzzocrea, D Impellizze, R Siracusa, R Di Paola Toxic Effects of Endocrine Disruptor Exposure on Collagen-Induced Arthritis Biomolecules, 2022-04-10;12(4):. 2022-04-10 [PMID: 35454153] (Mouse)
• RL Pope, A Chitrakar, P Sah, T Shadid, JD Ballard, LA Zenewicz Clostridioides difficile Toxin B Activates Group 3 Innate Lymphocytes Infection and Immunity, 2022;0(0):e0007322. 2022 [PMID: 35377172] (Rat)
• RL Pope, A Chitrakar, P Sah, T Shadid, JD Ballard, LA Zenewicz Clostridioides difficile Toxin B Activates Group 3 Innate Lymphocytes Infection and Immunity, 2022-04-04;0(0):e0007322. 2022-04-04 [PMID: 35377172] (Rat)
• IA Alsharif, HM Fayed, RF Abdel-Rahm, RM Abd-Elsala, HA Ogaly Miconazole Mitigates Acetic Acid-Induced Experimental Colitis in Rats: Insight into Inflammation, Oxidative Stress and Keap1/Nrf-2 Signaling Crosstalk Biology, 2022-02-13;11(2):. 2022-02-13 [PMID: 35205169] (Rat)
• HM Abuohashis, EH Zaghloul, AS El Sharkaw, EM Abbas, MM Ahmed, SS Al-Rejaie Pharmacological Effects of Marine-Derived Enterococcus faecium EA9 against Acute Lung Injury and Inflammation in Cecal Ligated and Punctured Septic Rats BioMed Research International, 2021-12-06;2021(0):5801700. 2021-12-06 [PMID: 34912891] (Rat)
• R Wang, J Liu, Z Wang, X Wu, H Guo, X Jiao, H Zhang, C Qi, X Li Mangiferin exert protective effects on joints of adjuvant-induced arthritis rats by regulating the MAPKs/NF-kappaB pathway of fibroblast-like synoviocytes International immunopharmacology, 2021-11-24;0(0):108352. 2021-11-24 [PMID: 34836794] (Rat)
• X Wang, B Yang, Y Li, J Luo, Y Wang AKR1C1 alleviates LPS?induced ALI in mice by activating the JAK2/STAT3 signaling pathway Molecular Medicine Reports, 2021-09-30;24(6):. 2021-09-30 [PMID: 34590152] (Mouse)
• CH Tsai, CT Pan, YY Chang, SY Peng, PC Lee, CW Liao, CT Shun, PT Li, VC Wu, CH Chou, IJ Tsai, CS Hung, YH Lin Aldosterone Excess Induced Mitochondria Decrease and Dysfunction via Mineralocorticoid Receptor and Oxidative Stress In Vitro and In Vivo Biomedicines, 2021-08-02;9(8):. 2021-08-02 [PMID: 34440149] (Human, Rat)
• Li Zhang, Bei Zhang, Linjing Wang, Mingwu Lou, Yunxia Shen Huanglian‑Houpo drug combination ameliorates H1N1‑induced mouse pneumonia via cytokines, antioxidant factors and TLR/MyD88/NF‑ kappa B signaling pathways Experimental and Therapeutic Medicine 2021-02-26 [PMID: 33747167] (Mouse)
• G Haji, CH Wiegman, C Michaeloud, MS Patel, K Curtis, P Bhavsar, MI Polkey, IM Adcock, KF Chung, COPDMAP co Mitochondrial dysfunction in airways and quadriceps muscle of patients with chronic obstructive pulmonary disease Respir Res, 2020-10-12;21(1):262. 2020-10-12 [PMID: 33046036] (Human)
• M Mohany, AZ Alanazi, F Alqahtani, OM Belali, MM Ahmed, SS Al-Rejaie LCZ696 mitigates diabetic-induced nephropathy through inhibiting oxidative stress, NF-kappaB mediated inflammation and glomerulosclerosis in rats PeerJ, 2020-06-19;8(0):e9196. 2020-06-19 [PMID: 32596035] (Rat)
• LR Ling, DH Zheng, ZY Zhang, WH Xie, YH Huang, ZX Chen, XZ Wang, D Li Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes Oncol Lett, 2020-02-17;19(4):2861-2869. 2020-02-17 [PMID: 32218840] (Mouse)
• SQ Rodríguez-, WA Trujillo-R, A Castillo-R, SE Totsuka-Su, TA Garcia-Cob, EG Cardona-Mu, AG Miranda-Dí, EJ Ramírez-Li, L García-Ben Effect of Telmisartan in the Oxidative Stress Components Induced by Ischemia Reperfusion in Rats Oxid Med Cell Longev, 2019-07-02;2019(0):1302985. 2019-07-02 [PMID: 31354899] (Rat)
• R Tan, Y He, S Zhang, D Pu, J Wu Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice J Ovarian Res, 2019-07-19;12(1):65. 2019-07-19 [PMID: 31324205] (Mouse)
• CH Chuang, CK Yang, PH Wu, Y Zhang, PJ Yang Acute renal injury induced by endotoxic shock in rats is alleviated via PI3K/Nrf2 pathway Eur Rev Med Pharmacol Sci, 2018-08-01;22(16):5394-5401. 2018-08-01 [PMID: 30178867] (Rat)
• X Meng, X Gao, Z Zhang, X Zhou, L Wu, M Yang, K Wang, H Ren, B Sun, T Wang Protective effect and mechanism of rat recombinant S100 calcium-binding protein A4 on oxidative stress injury of rat vascular endothelial cells Oncol Lett, 2018-07-11;16(3):3614-3622. 2018-07-11 [PMID: 30127969] (Rat)
• XN Wang, CJ Zhang, HL Diao, Y Zhang Protective Effects of Curcumin against Sodium Arsenite-induced Ovarian Oxidative Injury in a Mouse Model Chin. Med. J., 2017-05-05;130(9):1026-1032. 2017-05-05 [PMID: 28469096] (Mouse)
• Multigenerational Effects of Heavy Metals on Feeding, Growth, Initial Reproduction and Antioxidants in Caenorhabditis elegans PLoS ONE, 2016-04-26;11(4):e0154529. 2016-04-26 [PMID: 27116222] (Nematode)
• Mao S, Yang G, Li W, Zhang J, Liang H, Li J, Zhang M Gastroprotective Effects of Astragaloside IV against Acute Gastric Lesion in Rats. PLoS ONE, 2016-02-04;11(2):e0148146. 2016-02-04 [PMID: 26845156] (Rat)

FAQs for SOD2/Mn-SOD (DYC3419-2). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SOD2