Human M-CSF DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human M-CSF DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CSF1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human M-CSF DuoSet ELISA, 15 Plate

• colony stimulating factor 1 (macrophage)
• CSF1
• CSF-1
• Lanimostim
• macrophage colony stimulating factor
• macrophage colony-stimulating factor 1
• MCSF
• M-CSF
• MCSFlanimostim
• MGC31930

Background

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Publications for M-CSF (DY216)(20)

Publications using DY216

• Reina, J;Vallmajo-Martin, Q;Ning, J;Michi, AN;Yeung, K;Wahl, GM;Hunter, T; LHPP expression in triple-negative breast cancer promotes tumor growth and metastasis by modulating the tumor microenvironment bioRxiv : the preprint server for biology 2024-04-23 [PMID: 38712081] (Human)
• Surve, CR;Duran, CL;Ye, X;Chen, X;Lin, Y;Harney, AS;Wang, Y;Sharma, VP;Stanley, ER;McAuliffe, JC;Entenberg, D;Oktay, MH;Condeelis, JS; Signaling events at TMEM doorways provide potential targets for inhibiting breast cancer dissemination bioRxiv : the preprint server for biology 2024-01-09 [PMID: 38260319] (Mouse)
• Michielon, E;López González, M;Stolk, DA;Stolwijk, JGC;Roffel, S;Waaijman, T;Lougheed, SM;de Gruijl, TD;Gibbs, S; A Reconstructed Human Melanoma-in-Skin Model to Study Immune Modulatory and Angiogenic Mechanisms Facilitating Initial Melanoma Growth and Invasion Cancers 2023-05-20 [PMID: 37345186] (Human)
• DY Achkova, RE Beatson, J Maher CAR T-Cell Targeting of Macrophage Colony-Stimulating Factor Receptor Cells, 2022-07-13;11(14):. 2022-07-13 [PMID: 35883636] (Human)
• KN Lee, KH Park, YM Kim, I Cho, TE Kim Prediction of emergency cerclage outcomes in women with cervical insufficiency: The role of inflammatory, angiogenic, and extracellular matrix-related proteins in amniotic fluid PLoS ONE, 2022-05-10;17(5):e0268291. 2022-05-10 [PMID: 35536791] (Human)
• S Hakki, EJ Robinson, MG Robson Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula Scientific Reports, 2022-01-26;12(1):1427. 2022-01-26 [PMID: 35082332] (Human)
• Y Jung, M Son, YR Nam, J Choi, JR Heath, S Yang Microfluidic Single-Cell Proteomics Assay Chip: Lung Cancer Cell Line Case Study Micromachines, 2021-09-23;12(10):. 2021-09-23 [PMID: 34683198] (Human)
• IJ Yeh, SC Chen, MC Yen, YH Wu, CH Hung, PL Kuo 6-Shogaol Suppresses 2-Amino-1-Methyl-6-Phenylimidazo [4,5-b] Pyridine (PhIP)-Induced Human 786-O Renal Cell Carcinoma Osteoclastogenic Activity and Metastatic Potential Nutrients, 2019-09-28;11(10):. 2019-09-28 [PMID: 31569368] (Human)
• YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants Invest. Ophthalmol. Vis. Sci., 2019-09-03;60(12):3813-3820. 2019-09-03 [PMID: 31525777] (Human)
• P Dimou, RD Wright, KL Budge, A Midgley, SC Satchell, M Peak, MW Beresford The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis Sci Rep, 2019-06-06;9(1):8348. 2019-06-06 [PMID: 31171837] (Human)
• F Hua, Y Tian, Y Gao, C Li, X Liu Colony?stimulating factor 1 receptor inhibition blocks macrophage infiltration and endometrial cancer cell proliferation Mol Med Rep, 2019-02-18;0(0):. 2019-02-18 [PMID: 30816518] (Human)
• Hyung-Geun Moon, Seung-jae Kim, Jong Jin Jeong, Seon-Sook Han, Nizar N. Jarjour, Hyun Lee et al. Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization Immunity 2018-08-01 [PMID: 30054206] (Human)
• O Giricz, Y Mo, KB Dahlman, XM Cotto-Rios, C Vardabasso, H Nguyen, B Matusow, M Bartenstei, V Polishchuk, DB Johnson, TD Bhagat, R Shellooe, E Burton, J Tsai, C Zhang, G Habets, JM Greally, Y Yu, PA Kenny, GB Fields, K Pradhan, ER Stanley, E Bernstein, G Bollag, E Gavathioti, BL West, JA Sosman, AK Verma The RUNX1/IL-34/CSF-1R axis is an autocrinally regulated modulator of resistance to BRAF-V600E inhibition in melanoma JCI Insight, 2018-07-26;3(14):. 2018-07-26 [PMID: 30046005] (Human)
• E Ribechini, JA Hutchinson, S Hergovits, M Heuer, J Lucas, U Schleicher, AL Jordán Gar, SJ Potter, P Riquelme, H Brackmann, N Müller, H Raifer, I Berberich, M Huber, A Beilhack, M Lohoff, C Bogdan, M Eyrich, HM Hermanns, EK Geissler, MB Lutz Novel GM-CSF signals via IFN-?R/IRF-1 and AKT/mTOR license monocytes for suppressor function Blood Adv, 2017-06-07;1(14):947-960. 2017-06-07 [PMID: 29296736] (Human)
• Nijaguna M, Patil V, Urbach S, Shwetha S, Sravani K, Hegde A, Chandramouli B, Arivazhagan A, Marin P, Santosh V, Somasundaram K Glioblastoma-derived Macrophage Colony-stimulating Factor (MCSF) Induces Microglial Release of Insulin-like Growth Factor-binding Protein 1 (IGFBP1) to Promote Angiogenesis. J Biol Chem, 2015-08-05;290(38):23401-15. 2015-08-05 [PMID: 26245897] (Human)
• Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells. Br J Haematol, 2014-01-15;165(1):134-44. 2014-01-15 [PMID: 24428589] (Human)
• Lamprecht B, Walter K, Kreher S, Kumar R, Hummel M, Lenze D, Kochert K, Bouhlel MA, Richter J, Soler E, Stadhouders R, Johrens K, Wurster KD, Callen DF, Harte MF, Giefing M, Barlow R, Stein H, Anagnostopoulos I, Janz M, Cockerill PN, Siebert R, Dorken B, Bonifer C, Mathas S Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma. Nat. Med., 2010-05-02;16(5):571-9. 2010-05-02 [PMID: 20436485] (Human)
• Chang YC, Chen TC, Lee CT, Yang CY, Wang HW, Wang CC, Hsieh SL Epigenetic control of MHC class II expression in tumor-associated macrophages by decoy receptor 3. Blood, 2008-03-18;111(10):5054-63. 2008-03-18 [PMID: 18349319] (Human)
• Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells. Blood, 2007-09-11;110(13):4319-30. 2007-09-11 [PMID: 17848619] (Human)
• Merck E, Gaillard C, Scuiller M, Scapini P, Cassatella MA, Trinchieri G, Bates EE Ligation of the FcR gamma chain-associated human osteoclast-associated receptor enhances the proinflammatory responses of human monocytes and neutrophils. J. Immunol., 2006-03-01;176(5):3149-56. 2006-03-01 [PMID: 16493074] (Human)

FAQs for M-CSF (DY216). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CSF1