Human LDLR DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human LDLR DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: LDLR

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human LDLR DuoSet ELISA, 15 Plate

• FH
• FHC
• LDL R
• LDL receptor
• LDLCQ2
• LDLR
• low density lipoprotein receptor
• low-density lipoprotein receptor class A domain-containing protein 3
• low-density lipoprotein receptor

Background

LDL R (Low Density Lipoprotein Receptor) is a widely expressed cell surface scavenger receptor. LDL R binds ApoB and ApoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively. Hepatocyte LDL R is responsible for endocytosis and clearing of most plasma LDL cholesterol. At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDL R to the cell surface. Lack of LDL R expression or function causes familial hypercholesterolemia (FH). The protease PCSK9 can also cause increased plasma cholesterol by promoting LDL R degradation rather than recycling to the cell surface.

Publications for LDLR (DY2148)(4)

Publications using DY2148

• A Alabi, XD Xia, HM Gu, F Wang, SJ Deng, N Yang, A Adijiang, DN Douglas, NM Kneteman, Y Xue, L Chen, S Qin, G Wang, DW Zhang Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis Nature Communications, 2021-03-25;12(1):1889. 2021-03-25 [PMID: 33767172] (Human)
• Delia Susan-Resi A novel mechanism causing familial hypercholesterolemia: The proprotein convertase subtilisin/kexin type 9-resistant Arg410Ser LDL receptor mutation J. Biol. Chem, 2016-12-20;0(0):. 2016-12-20 [PMID: 27998977] (Human)
• Elodie Weider Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation J Biol Chem, 2016-06-08;0(0):. 2016-06-08 [PMID: 27284008] (Human)
• Nagiec M, Skepner A, Negri J, Eichhorn M, Kuperwasser N, Comer E, Muncipinto G, Subramanian A, Clish C, Musunuru K, Duvall J, Foley M, Perez J, Palmer M Modulators of hepatic lipoprotein metabolism identified in a search for small-molecule inducers of tribbles pseudokinase 1 expression. PLoS ONE, 2015-03-26;10(3):e0120295. 2015-03-26 [PMID: 25811180] (Human)

FAQs for LDLR (DY2148). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: LDLR