Human Kininogen DuoSet ELISA, 5 Plate Summary
| Source |
N/A |
| Assay Type |
Solid Phase Sandwich ELISA |
| Inter-Assay |
|
| Intra-Assay |
|
| Spike Recovery |
|
| Sample Volume |
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
No significant interference observed with available related molecules. |
Packaging, Storage & Formulations
| Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human Kininogen DuoSet ELISA, 5 Plate
Background
Kininogen, also known as 2-thiol proteinase inhibitor, is a multi-functional protein. The high molecule weight (HMW) form of kininogen is synthesized as a precursor protein (amino acid residues 1 to 644). The mature chain (residues 19 to 644) is further processed into the heavy chain (residues 19 to 380) and the light chain (residues 390 to 644) with release of the active peptide bradykinin. The heavy chain consists of three cystatin-like domains, which inhibit cysteine proteases. The light chain consists of a His-rich domain, which is associated with clotting activity.
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Product General Protocols
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FAQs for Kininogen (DY1569-05). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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