Human IL-8/CXCL8 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Human IL-8/CXCL8 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
CXCL8

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DY208 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-8/CXCL8 DuoSet ELISA, 15 Plate

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • CXCL8SCYB8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MDNCFb-ENAP
  • member 8
  • MONAP
  • MONAPGCP1
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1

Background

Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent. 

Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells. 

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1. 

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

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Publications for CXCL8/IL-8 (DY208)(379)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Guinea Pig, N/A.

We have publications tested in 1 application: ChIP.


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ChIP
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Human
(363)
Mouse
(2)
Rat
(1)
Bovine
(5)
Guinea Pig
(3)
N/A
(1)
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Showing Publications 1 - 10 of 379. Show All 379 Publications.
Publications using DY208 Applications Species
LAC Teixeira, JM Dos Santos, AN Parentoni, LP Lima, TC Duarte, FP Brant, CDC Neves, FSM Pereira, NCP Avelar, AL Danielewic, AAO Leopoldino, SP Costa, AN Arrieiro, LA Soares, ACN Prates, JNP Nobre, A de Carvalh, VC de Oliveir, MX Oliveira, PH Scheidt Fi, HS Costa, V Amaral Men, R Taiar, AC Rodrigues Adiponectin Is a Contributing Factor of Low Appendicular Lean Mass in Older Community-Dwelling Women: A Cross-Sectional Study Journal of Clinical Medicine, 2022;11(23):. 2022 [PMID: 36498747] (Human) Human
T Luong, E Cukierman Eribulin normalizes pancreatic cancer-associated fibroblasts by simulating selected features of TGFbeta inhibition BMC Cancer, 2022;22(1):1255. 2022 [PMID: 36461015] (Human) Human
J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer Cancer Research, 2022;0(0):OF1-OF14. 2022 [PMID: 36306422] (Human) Human
AA Lozhkov, MA Plotnikova, MA Egorova, IL Baranovska, EA Elpaeva, SA Klotchenko, AV Vasin Simultaneous Detection of RIG-1, MDA5, and IFIT-1 Expression Is a Convenient Tool for Evaluation of the Interferon-Mediated Response Viruses, 2022;14(10):. 2022 [PMID: 36298646] (Human) Human
L Zuliani-Al, ML Govasli, J Rasaiyaah, C Monit, SO Perry, RP Sumner, S McAlpine-S, C Dickson, KM Rifat Fays, L Hilditch, RJ Miles, F Bibollet-R, BH Hahn, T Boecking, N Pinotsis, LC James, DA Jacques, GJ Towers Evasion of cGAS and TRIM5 defines pandemic HIV Nature Microbiology, 2022;7(11):1762-1776. 2022 [PMID: 36289397] (Human) Human
E Fournier, M Leveque, P Ruiz, J Ratel, C Durif, S Chalancon, F Amiard, M Edely, V Bezirard, E Gaultier, B Lamas, E Houdeau, F Lagarde, E Engel, L Etienne-Me, S Blanquet-D, M Mercier-Bo Microplastics: What happens in the human digestive tract? First evidences in adults using in vitro gut models Journal of Hazardous Materials, 2023;442(0):130010. 2023 [PMID: 36182891] (Human) Human
M Patankar, M Li, A Khalatbari, JD Castle, L Hu, C Zhang, A Shaker Inflammatory and Proliferative Pathway Activation in Human Esophageal Myofibroblasts Treated with Acidic Bile Salts International Journal of Molecular Sciences, 2022;23(18):. 2022 [PMID: 36142285] (Human) Human
P Marona, J Górka, O Kwapisz, J Jura, J Rys, RM Hoffman, K Miekus Resistance to tyrosine kinase inhibitors promotes renal cancer progression through MCPIP1 tumor-suppressor downregulation and c-Met activation Cell Death & Disease, 2022;13(9):814. 2022 [PMID: 36138026] (Human) Human
L Sansone, A de Iure, M Cristina, M Belli, L Vitiello, F Marcolongo, A Rosellini, L Macera, PG Spezia, C Tomino, S Bonassi, MA Russo, F Maggi, P Russo Nicotine in Combination with SARS-CoV-2 Affects Cells Viability, Inflammatory Response and Ultrastructural Integrity International Journal of Molecular Sciences, 2022;23(16):. 2022 [PMID: 36012747] (Human) Human
C Amormino, V Tedeschi, G Paldino, S Arcieri, MT Fiorillo, A Paiardini, L Tuosto, M Kunkl SARS-CoV-2 Spike Does Not Possess Intrinsic Superantigen-like Inflammatory Activity Cells, 2022;11(16):. 2022 [PMID: 36010602] (Human) Human
Show All 379 Publications.

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Bioinformatics

Gene Symbol CXCL8