Human IL-5 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-5 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL5

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-5 DuoSet ELISA, 15 Plate

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Publications for IL-5 (DY205)(28)

Publications using DY205

• Navas, A;Matzaraki, V;van Eekeren, LE;Blaauw, MJT;Groenendijk, AL;Vos, WAJW;Jacobs-Cleophas, M;Dos Santos, JC;van der Ven, AJAM;Joosten, LAB;Netea, MG; Plasma Proteomic Signature as a Predictor of Age Advancement in People Living With HIV Aging cell 2025-01-15 [PMID: 39812213] (Human)
• Katsoulis, O;Toussaint, M;Jackson, MM;Mallia, P;Footitt, J;Mincham, KT;Meyer, GFM;Kebadze, T;Gilmour, A;Long, M;Aswani, AD;Snelgrove, RJ;Johnston, SL;Chalmers, JD;Singanayagam, A; Neutrophil extracellular traps promote immunopathogenesis of virus-induced COPD exacerbations Nature communications 2024-07-09 [PMID: 38982052] (Human)
• da Costa Teixeira, LA;Soares, LA;Lima, LP;Avelar, NCP;de Moura, JA;Leopoldino, AAO;Figueiredo, PHS;Parentoni, AN;Mendonça, VA;Lacerda, ACR; Cognitive function is associated with performance in time up and go test and with leptin blood levels in community-dwelling older women Scientific reports 2024-04-29 [PMID: 38684691] (Human)
• Augusto da Costa Teixeira, L;Rocha-Vieira, E;Aparecida Soares, L;Mota de Oliveira, F;Aparecida Oliveira Leopoldino, A;Netto Parentoni, A;Amaral Mendonça, V;Cristina Rodrigues Lacerda, A; The strong inverse association between plasma concentrations of soluble tumor necrosis factor receptors type 1 with adiponectin/leptin ratio in older women Cytokine 2024-01-27 [PMID: 38281360] (Human)
• Wu, WC;Shiu, C;Tong, TK;Leung, SO;Hui, CW; Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune Diseases Journal of immunology research 2023-12-08 [PMID: 38106519] (Human)
• Nikiforov, NG;Kirichenko, TV;Kubekina, MV;Chegodaev, YS;Zhuravlev, AD;Ilchuk, LA;Nikolaeva, MA;Arefieva, AS;Popov, MA;Verkhova, SS;Bagheri Ekta, M;Orekhov, AN; Macrophages derived from LPS-stimulated monocytes from individuals with subclinical atherosclerosis were characterized by increased pro-inflammatory activity Cytokine 2023-12-01 [PMID: 37918051] (Human)
• da Costa Teixeira, LA;Avelar, NCP;Peixoto, MFD;Parentoni, AN;Santos, JMD;Pereira, FSM;Danielewicz, AL;Leopoldino, AAO;Costa, SP;Arrieiro, AN;Soares, LA;da Silva Lage, VK;Prates, ACN;Taiar, R;de Carvalho Bastone, A;Oliveira, VC;Oliveira, MX;Costa, HS;Nobre, JNP;Brant, FP;Duarte, TC;Figueiredo, PHS;Mendonça, VA;Lacerda, ACR; Inflammatory biomarkers at different stages of Sarcopenia in older women Scientific reports 2023-06-26 [PMID: 37365209] (Human)
• LAC Teixeira, JM Dos Santos, AN Parentoni, LP Lima, TC Duarte, FP Brant, CDC Neves, FSM Pereira, NCP Avelar, AL Danielewic, AAO Leopoldino, SP Costa, AN Arrieiro, LA Soares, ACN Prates, JNP Nobre, A de Carvalh, VC de Oliveir, MX Oliveira, PH Scheidt Fi, HS Costa, V Amaral Men, R Taiar, AC Rodrigues Adiponectin Is a Contributing Factor of Low Appendicular Lean Mass in Older Community-Dwelling Women: A Cross-Sectional Study Journal of Clinical Medicine, 2022-12-02;11(23):. 2022-12-02 [PMID: 36498747] (Human)
• KEC Blokland, M Nizamoglu, H Habibie, T Borghuis, M Schuliga, BN Melgert, DA Knight, CA Brandsma, SD Pouwels, JK Burgess Substrate stiffness engineered to replicate disease conditions influence senescence and fibrotic responses in primary lung fibroblasts Frontiers in Pharmacology, 2022-11-03;13(0):989169. 2022-11-03 [PMID: 36408252] (Human)
• J Feldt, AJ Donaubauer, J Welss, U Schneider, US Gaipl, F Paulsen Anti-inflammatory effects of an autologous gold-based serum therapy in osteoarthritis patients Scientific Reports, 2022-03-03;12(1):3560. 2022-03-03 [PMID: 35241691] (Human)
• SL Mathiasen, L Gall-Mas, IS Pateras, SDP Theodorou, MRJ Namini, MB Hansen, OCB Martin, CK Vadivel, K Ntostoglou, D Butter, M Givskov, C Geisler, AN Akbar, VG Gorgoulis, T Frisan, N Ødum, T Krejsgaard Bacterial genotoxins induce T�cell senescence Cell Reports, 2021-06-08;35(10):109220. 2021-06-08 [PMID: 34107253] (Human)
• M Ueno, I Miyagawa, K Nakano, S Iwata, K Hanami, S Fukuyo, S Kubo, Y Miyazaki, A Kawabe, H Yoshinari, S Nakayamada, Y Tanaka Effectiveness and safety of mepolizumab in combination with corticosteroids in patients with eosinophilic granulomatosis with polyangiitis Arthritis Research & Therapy, 2021-03-16;23(1):86. 2021-03-16 [PMID: 33726827] (Human)
• RP Madeira, LM Dal'Mas Ro, P de Cássia, C Mady, BM Ianni, AC Torrecilha New Biomarker in Chagas Disease: Extracellular Vesicles Isolated from Peripheral Blood in Chronic Chagas Disease Patients Modulate the Human Immune Response Journal of Immunology Research, 2021-01-11;2021(0):6650670. 2021-01-11 [PMID: 33506056] (Human)
• GR Kathamuthu, K Moideen, R Sridhar, D Baskaran, S Babu Altered plasma levels of &betaC and &gammaC chain cytokines and post-treatment modulation in tuberculous lymphadenitis Cytokine, 2020-12-17;138(0):155405. 2020-12-17 [PMID: 33341600] (Human)
• I Patlan, MP Jeffrey, D Lewis, J Green-John, S Dogra Salivary IL-8 and IL-1RA changes induced by exercise in cold-dry and normal conditions Cytokine, 2020-10-28;137(0):155347. 2020-10-28 [PMID: 33128925] (Human)
• Rui Zheng, Yang Chen, Jianbo Shi, Kai Wang, Xuekun Huang, Yueqi Sun et al. Combinatorial IL-17RB, ST2, and TSLPR Signaling in Dendritic Cells of Patients With Allergic Rhinitis Frontiers in Cell and Developmental Biology 2020-04-03 [PMID: 32309281] (Human)
• C Malmhäll, K Johansson, C Winkler, S Alawieh, L Ekerljung, M Rådinger Altered miR-155 expression in Allergic Asthmatic airways Scand. J. Immunol, 2017-04-01;0(0):. 2017-04-01 [PMID: 28199728] (Human)
• Genetic, immunological, and clinical features of patients with bacterial and fungal infections due to inherited IL-17RA deficiency Proc. Natl. Acad. Sci. U.S.A, 2016-12-07;0(0):. 2016-12-07 [PMID: 27930337] (Human)
• Liukko A, Kinnunen T, Rytkonen-Nissinen M, Kailaanmaki A, Randell J, Maillere B, Virtanen T Human CD4+ T cell responses to the dog major allergen Can f 1 and its human homologue tear lipocalin resemble each other. PLoS ONE, 2014-05-29;9(5):e98461. 2014-05-29 [PMID: 24875388] (Human)
• Arndts , Kathrin, Specht , Sabine, Debrah , Alexande, Tamarozzi , Francesc, Klarmann Schulz , Ute, Mand , Sabine, Batsa , Linda, Kwarteng , Alexande, Taylor , Mark, Adjei , Ohene, Martin , Coralie, Layland , Laura E, Hoerauf , Achim Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels. PLoS Negl Trop Dis, 2014-02-20;8(2):e2679. 2014-02-20 [PMID: 24587458] (Human)
• Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation. Eur J Immunol, 2014-02-18;44(5):1330-40. 2014-02-18 [PMID: 24549985] (Human)
• Boisson B, Wang C, Pedergnana V, Wu L, Cypowyj S, Rybojad M, Belkadi A, Picard C, Abel L, Fieschi C, Puel A, Li X, Casanova J An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis. Immunity, 2013-10-10;39(4):676-86. 2013-10-10 [PMID: 24120361] (Human)
• Arndts K, Deininger S, Specht S, Klarmann U, Mand S, Adjobimey T, Debrah AY, Batsa L, Kwarteng A, Epp C, Taylor M, Adjei O, Layland LE, Hoerauf A Elevated adaptive immune responses are associated with latent infections of Wuchereria bancrofti. PLoS Negl Trop Dis, 2012-04-03;6(4):e1611. 2012-04-03 [PMID: 22509424] (Human)
• Ramana Rao PV, Rajasekaran S, Raja A Natural killer cell-mediated cytokine response among HIV-positive south Indians with pulmonary tuberculosis. J. Interferon Cytokine Res., 2010-01-01;30(1):33-42. 2010-01-01 [PMID: 20028198] (Human)
• Lamhamedi-Cherradi SE, Martin RE, Ito T, Kheradmand F, Corry DB, Liu YJ, Moyle M Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12. J. Immunol., 2008-05-01;180(9):6000-9. 2008-05-01 [PMID: 18424720] (Human)
• Nassenstein C, Kammertoens T, Veres TZ, Uckert W, Spies E, Fuchs B, Krug N, Braun A Neuroimmune crosstalk in asthma: dual role of the neurotrophin receptor p75NTR. J. Allergy Clin. Immunol., 2007-08-22;120(5):1089-96. 2007-08-22 [PMID: 17716721] (Mouse)
• Cox AL, Coles AJ, Nortje J, Bradley PG, Chatfield DA, Thompson SJ, Menon DK An investigation of auto-reactivity after head injury. J. Neuroimmunol., 2006-03-06;174(1):180-6. 2006-03-06 [PMID: 16519951] (Human)
• Freyne EJ, Lacrampe JF, Deroose F, Boeckx GM, Willems M, Embrechts W, Coesemans E, Willems JJ, Fortin JM, Ligney Y, Dillen LL, Cools WF, Goossens J, Corens D, De Groot A, Van Wauwe JP Synthesis and biological evaluation of 1,2,4-triazinylphenylalkylthiazolecarboxylic acid esters as cytokine-inhibiting antedrugs with strong bronchodilating effects in an animal model of asthma. J. Med. Chem., 2005-03-24;48(6):2167-75. 2005-03-24 [PMID: 15771459] (Human)

FAQs for IL-5 (DY205). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL5