Home » IL-38/IL-1F10 » IL-38/IL-1F10 ELISA Kits » Human IL-38/IL-1F10 DuoSet ELISA, 5 Plate

Human IL-38/IL-1F10 DuoSet ELISA, 5 Plate

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Human IL-38/IL-1F10 DuoSet ELISA, 5 Plate Summary

Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY9110-05.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-38/IL-1F10 DuoSet ELISA, 5 Plate

  • FIL1- theta
  • FKSG75
  • IL1F10
  • IL-1HY2
  • IL38
  • IL-38
  • interleukin 1 family, member 10 (theta)
  • UNQ6119/PRO20041

Background

Human IL-38/IL-1F10 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-38 shares 82% aa sequence identity with mouse IL-1F10. Human IL-38 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 beta, respectively.

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1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.
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1 μg/lane of Recombinant HumanIL-1 alpha /IL-1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.
Recombinant Human IL-1 alpha /IL-1F1 (Catalog # 200-LA/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 1-6 pg/mL.
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IL‑1 RI was detected in perfusion fixed frozen sections of mouse skin (tail) using Goat Anti-Mouse IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND50 is typically 0.2-1.0 µg/mL.
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IL-36Ra/IL-1F5
[Unconjugated]
1275-IL/CF
Recombinant Human IL-36Ra/IL-1F5 (Catalog # 1275-IL/CF) inhibits Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB) induced IL-8 secretion by A431 human epithelial carcinoma cells. The ED50 for this effect is 0.2-1 μg/mL.
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Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND50 is typically0.08-0.4 µg/mL.
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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IL-36 beta/IL-1F8
Antibody [Unconjugate ...
AF1099
Recombinant Human IL‑36 beta /IL‑1F8 (aa 5-157) (Catalog # 6834-IL) induces IL-8 secretion in human pre-adipocytes in a dose-dependent manner (orange line). IL-8 Secretion elicited by Recombinant Human IL‑36 beta /IL‑1F8 (aa 5-157) (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑36 beta /IL‑1F8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1099). The ND50 is typically 50‑300 ng/mL.
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IL-22
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782-IL/CF
1 µg/lane of Recombinant Human IL-22 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-22 (Catalog # 782‑IL/CF) induces IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. The ED50 for this effect is 60‑300 pg/mL.
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CSRP1 Antibody
AF5739
Western blot shows lysates of ME-180 human cervical epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat CSRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CSRP1, CSRP2, CSRP3 (5 ng/lane) were included. A specific band for CSRP1 was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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14-3-3 zeta
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MAB2669
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14‑3‑3 zeta  was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to nuclei and plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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DCDL40
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AF872
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NBP2-19860
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Western Blot: Plastin L Antibody [NBP2-19860] - arious whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000.
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HNF1 Antibody
NBP1-33596
Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: HNF1 Antibody [NBP1-33596] - Analysis. Mouse tissue extracts (50 Ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000.
Western Blot: HNF1 Antibody [NBP1-33596] - Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000.
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NBP2-67150
Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.
Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.
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Publications for IL-38/IL-1F10 (DY9110-05)(4)

We have publications tested in 1 confirmed species: Human.


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