Human IL-1 RII Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-1 RII Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-1 sRII Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-1 sRII in serum, plasma, and cell culture supernates. It contains insect cell-expressed recombinant human IL-1 sRII and has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-1 sRII showed linear curves that were parallel to the standard curves obtained... using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 sRII. The presence of high levels of IL-1 beta (> 7.5 ng/mL) in samples has been found to produce some interference (> 10% change) when assaying samples containing IL-1 sRII in the mid-level range.
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• Specificity: Natural and recombinant human IL-1 sRII
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL1R2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1 RII Quantikine ELISA Kit

• beta
• CD121b antigen
• CD121b
• IL-1 R beta
• IL-1 RII
• IL1R2
• IL1RBCD121 antigen-like family member B
• IL-1R-beta
• IL1RII
• IL-1RII
• IL-1RT2
• IL-1RT-2
• interleukin 1 receptor, type II
• Interleukin-1 receptor beta
• MGC47725

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and, endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

IL-1 RII has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-1 RII (DR1B00)(16)

Publications using DR1B00

• J Lin, Q Li, T Jin, J Wang, Y Gong, Q Lv, M Wang, J Chen, M Shang, Y Zhao, G Fu Cardiomyocyte IL-1R2 protects heart from ischemia/reperfusion injury by attenuating IL-17RA-mediated cardiomyocyte apoptosis Cell Death & Disease, 2022-01-27;13(1):90. 2022-01-27 [PMID: 35087030] (Human)
• M Yuan, L Wang, H Huang, Y Li, X Zheng, Q Shao, J Jiang IL-1R2 expression in human gastric cancer and its clinical significance Bioscience Reports, 2021-03-26;0(0):. 2021-03-26 [PMID: 33704402] (Human)
• TAT Tran, HW Grievink, K Lipinska, C Kluft, J Burggraaf, M Moerland, D Tasev, KE Malone Whole blood assay as a model for in vitro evaluation of inflammasome activation and subsequent caspase-mediated interleukin-1 beta release PLoS ONE, 2019-04-08;14(4):e0214999. 2019-04-08 [PMID: 30958862] (Human)
• Pathak S, Goldofsky E, Vivas EX, Bonagura VR, Vambutas A IL-1beta is overexpressed and aberrantly regulated in corticosteroid nonresponders with autoimmune inner ear disease. J. Immunol., 2011-01-03;186(3):1870-9. 2011-01-03 [PMID: 21199898] (Human)
• Harboe E, Tjensvoll AB, Vefring HK, Goransson LG, Kvaloy JT, Omdal R Fatigue in primary Sj0gren's syndrome--a link to sickness behaviour in animals? Brain Behav. Immun., 2009-06-26;23(8):1104-8. 2009-06-26 [PMID: 19560535] (Human)
• Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes. J. Exp. Med., 2009-04-13;206(5):1029-36. 2009-04-13 [PMID: 19364880] (Human)
• Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes. Blood, 2007-12-10;111(4):2339-46. 2007-12-10 [PMID: 18070985] (Human)
• Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Br. J. Pharmacol., 2007-03-12;151(1):115-27. 2007-03-12 [PMID: 17351655] (Human)
• Lindberg C, Chromek M, Ahrengart L, Brauner A, Schultzberg M, Garlind A Soluble interleukin-1 receptor type II, IL-18 and caspase-1 in mild cognitive impairment and severe Alzheimer's disease. Neurochem. Int., 2005-06-01;46(7):551-7. 2005-06-01 [PMID: 15843049] (Human)
• Kaneyama K, Segami N, Sun W, Sato J, Fujimura K Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-04-01;99(4):411-8. 2005-04-01 [PMID: 15772591] (Human)
• Tominaga K, Habu M, Sukedai M, Hirota Y, Takahashi T, Fukuda J IL-1 beta, IL-1 receptor antagonist and soluble type II IL-1 receptor in synovial fluid of patients with temporomandibular disorders. Arch. Oral Biol., 2004-06-01;49(6):493-9. 2004-06-01 [PMID: 15099807] (Human)
• Brooks-Asplund EM, Tupper CE, Daun JM, Kenney WL, Cannon JG Determinants of plasma leptin concentration in postmenopausal women. Mech. Ageing Dev., 2004-02-01;125(2):117-9. 2004-02-01 [PMID: 15037014] (Human)
• Bronge L, Wahlund LO, Brauner A, Basun H, Garlind A White matter lesions and soluble interleukin-1 receptor type II in CSF from demented and non-demented subjects. Neurochem. Int., 2002-10-01;41(4):217-22. 2002-10-01 [PMID: 12106772] (Human)
• Cubitt CL, Lausch RN, Oakes JE Synthesis of type II interleukin-1 receptors by human corneal epithelial cells but not by keratocytes. Invest. Ophthalmol. Vis. Sci., 2001-03-01;42(3):701-4. 2001-03-01 [PMID: 11222530] (Human)
• Attur MG, Dave MN, Clancy RM, Patel IR, Abramson SB, Amin AR Functional genomic analysis in arthritis-affected cartilage: yin-yang regulation of inflammatory mediators by alpha 5 beta 1 and alpha V beta 3 integrins. J. Immunol., 2000-03-01;164(5):2684-91. 2000-03-01 [PMID: 10679109] (Human)
• Estrogen’s bone-protective effects may involve differential IL-1 receptor regulation in human osteoclast-like cells [PMID: 10330423] (Human)

Bioinformatics

• Gene Symbol: IL1R2