Human FABP4 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human FABP4 Quantikine ELISA Kit Summary

• Background: The Quantikine Human FABP4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FABP4 in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant human FABP4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FABP4 showed linear curves that were parallel to the standard... curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FABP4.
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• Specificity: Natural and recombinant human FABP4.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FABP4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human FABP4 Quantikine ELISA Kit

• Adipocyte lipid-binding protein
• Adipocyte-type fatty acid-binding protein
• AFABP
• A-FABP
• A-FABPAFABP
• ALBP
• aP2
• FABP4
• fatty acid binding protein 4, adipocyte
• Fatty acid-binding protein 4
• fatty acid-binding protein, adipocyte

Background

FABP4 (Fatty Acid Binding Protein 4), also known as A-FABP and aP2, is the predominant FABP found in adipocytes and is often used as a marker for adipocyte differentiation. It is also expressed in macrophages, dendritic cells, and endothelial cells. FABP4 is a key mediator of intracellular fatty acid transport and metabolism in adipose tissue. Its expression is regulated by multiple factors including fatty acids, PPAR gamma agonists, and Insulin, and its levels increase with lipolytic stimulation. It can increase the hydrolytic activity of Hormone-Sensitive Lipase and increase the production of proinflammatory cytokines and chemokines. FABP4 upregulation in adipocytes and macrophages is associated with the development of Insulin resistance, hypertriacylglycerolaemia, and atherosclerosis. Serum levels of FABP4 are elevated in obesity and metabolic syndrome, type 2 diabetes, HIV-associated lipodystrophy, polycystic ovary syndrome, nonalcoholic fatty liver disease, atherosclerosis, and acute ischaemic stroke. FABP4 is also overexpressed in multiple cancer types including ovarian, bladder, glioblastoma, and oral, and it may play a role in tumor progression.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for FABP4/A-FABP (DFBP40)(14)

Publications using DFBP40

• Gu, Y;Avolio, E;Alvino, VV;Thomas, AC;Herman, A;Miller, PJ;Sullivan, N;Faulkner, A;Madeddu, P; The tyrosine kinase inhibitor Dasatinib reduces cardiac steatosis and fibrosis in obese, type 2 diabetic mice Cardiovascular diabetology 2023-08-17 [PMID: 37592236] (Human)
• H Svensson, S Einarsson, D Olausson, L Kluge, C Bergh, S Edén, M Lönn, A Thurin-Kje Inflammatory and metabolic markers in relation to outcome of in vitro fertilization in a cohort of predominantly overweight and obese women Scientific Reports, 2022-08-03;12(1):13331. 2022-08-03 [PMID: 35922472] (Human)
• K Miniewska, K Maliszewsk, K Pietrowska, J Godzie?, ? ?abieniec, M Mojsak, A Kr?towski, M Ciborowski PET/MRI-Evaluated Activation of Brown Adipose Tissue via Cold Exposure Impacts Lipid Metabolism Metabolites, 2022-05-19;12(5):. 2022-05-19 [PMID: 35629960] (Human)
• I Ron, RK Lerner, M Rathaus, R Livne, S Ron, E Barhod, R Hemi, A Tirosh, T Strauss, K Ofir, I Goldstein, IM Pessach, A Tirosh The adipokine FABP4 is a key regulator of neonatal glucose homeostasis JCI Insight, 2021-10-22;6(20):. 2021-10-22 [PMID: 34676825] (Human)
• H Wang, J Cao, JB Su, XQ Wang, X Wang, DM Zhang, XH Wang Serum fatty acid-binding protein 4 levels and responses of pancreatic islet &beta-cells and &alpha-cells in patients with type 2 diabetes Diabetology & metabolic syndrome, 2021-06-26;13(1):70. 2021-06-26 [PMID: 34174950] (Human)
• D Tsakogiann, E Kalogera, F Zagouri, E Zografos, D Balalis, G Bletsa Determination of FABP4, RBP4 and the MMP-9/NGAL complex in the serum of women with breast cancer Oncology Letters, 2020-12-04;21(2):85. 2020-12-04 [PMID: 33376518] (Human)
• T Sarabhai, S Kahl, J Szendroedi, DF Markgraf, OP Zaharia, C Barosa, C Herder, F Wickrath, P Bobrov, JH Hwang, JG Jones, M Roden Monounsaturated fat rapidly induces hepatic gluconeogenesis and whole-body insulin resistance JCI Insight, 2020-05-21;5(10):. 2020-05-21 [PMID: 32434996] (Human)
• YZ Chen, SQ Zhou, YQ Chen, H Peng, YG Zhuang Plasma Adipokines in Patients Resuscitated from Cardiac Arrest: Difference of Visfatin between Survivors and Nonsurvivors Dis. Markers, 2020-01-14;2020(0):9608276. 2020-01-14 [PMID: 32015774] (Human)
• S Numao, Y Nagasawa, N Goromaru, S Tamaki Comparison of plasma fatty acid binding protein 4 concentration in venous and capillary blood PLoS ONE, 2019-12-11;14(12):e0226374. 2019-12-11 [PMID: 31826012] (Human)
• J Patro-Ma?y, M Trojnar, ? Kimber-Tro, R Mierzy?ski, J Bartosiewi, J Oleszczuk, B Leszczy?sk FABP4 in Gestational Diabetes-Association between Mothers and Offspring J Clin Med, 2019-02-27;8(3):. 2019-02-27 [PMID: 30818771] (Human)
• O Huttala, M Palmroth, P Hemminki, T Toimela, T Heinonen, T Ylikomi, JR Sarkanen Development of Versatile Human In Vitro Vascularized Adipose Tissue Model with Serum-Free Angiogenesis and Natural Adipogenesis Induction Basic Clin. Pharmacol. Toxicol., 2018-04-27;0(0):. 2018-04-27 [PMID: 29443452] (Human)
• C Jahansouz, H Xu, AV Hertzel, S Kizy, KA Steen, R Foncea, FJ Serrot, N Kvalheim, G Luthra, K Ewing, DB Leslie, S Ikramuddin, DA Bernlohr Partitioning of adipose lipid metabolism by altered expression and function of PPAR isoforms after bariatric surgery Int J Obes (Lond), 2017-08-14;42(2):139-146. 2017-08-14 [PMID: 28894292] (Human)
• I Graupera, M Coll, E Pose, C Elia, S Piano, E Solà, D Blaya, P Huelin, C Solé, R Moreira, G de Prada, N Fabrellas, A Juanola, M Morales-Ru, P Sancho-Bru, C Villanueva, P Ginès Adipocyte Fatty-Acid Binding Protein is Overexpressed in Cirrhosis and Correlates with Clinical Outcomes Sci Rep, 2017-05-12;7(1):1829. 2017-05-12 [PMID: 28500294] (Human)
• Leryn J Reynolds Using Neonatal Skin to Study the Developmental Programming of Aging Exp. Gerontol, 2016-12-27;0(0):. 2016-12-27 [PMID: 28034763] (Human)

FAQs for FABP4/A-FABP (DFBP40). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FABP4