Human eNOS DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human eNOS DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: NOS3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human eNOS DuoSet ELISA, 5 Plate

• Constitutive NOS
• EC 1.14.13.39
• ECNOS
• Endothelial NOS
• endothelial
• eNOS
• eNOScNOS
• nitric oxide synthase 3 (endothelial cell)
• NOS type III
• NOS3
• NOSIII

Background

Nitric oxide (NO) is a pleiotropic signaling molecule implicated in diverse biological processes including inhibition of platelet aggregation, regulation of neurotransmission, vasodilation, immune responses, and inflammation. NO is synthesized from L-arginine and O2 by three nitric oxide synthase (NOS) enzymes termed endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS). Each enzyme isoform is expressed in a variety of tissues and cell types. While eNOS and nNOS generally exhibit constitutive expression and are involved in physiological signaling and maintenance functions, iNOS expression is induced by inflammatory stimuli and is associated with both normal and pathological immune responses.

Publications for eNOS (DY950-05)(5)

Publications using DY950-05

• Ren, J;Ren, B;Fu, T;Ma, Y;Tan, Y;Zhang, S;Li, Y;Wang, Q;Chang, X;Tong, Y; Pyruvate kinase M2 sustains cardiac mitochondrial integrity in septic cardiomyopathy by regulating PHB2-dependent mitochondrial biogenesis International journal of medical sciences 2024-04-08 [PMID: 38774750] (Mouse)
• Martín-Núñez, E;Goñi-Olóriz, M;Matilla, L;Garaikoetxea, M;Mourino-Alvarez, L;Navarro, A;Fernández-Celis, A;Tamayo, I;Gainza, A;Álvarez, V;Sádaba, R;Barderas, MG;Jover, E;López-Andrés, N; Influence of diabetes mellitus on the pathological profile of aortic stenosis: a sex-based approach Cardiovascular diabetology 2023-10-17 [PMID: 37848892] (Human)
• Ponasenko, A;Sinitskaya, A;Sinitsky, M;Khutornaya, M;Barbarash, O; The Role of Polymorphism in the Endothelial Homeostasis and Vitamin D Metabolism Genes in the Severity of Coronary Artery Disease Biomedicines 2023-08-25 [PMID: 37760823] (Human)
• K Kowalska, DE Habrowska-, C Neumayer, M Bolliger, C Domenig, AW Piastowska, I Huk, A Piechota-P Lower levels of Caveolin-1 and higher levels of endothelial nitric oxide synthase are observed in abdominal aortic aneurysm patients treated with simvastatin Acta Biochim. Pol., 2018-03-15;65(1):111-118. 2018-03-15 [PMID: 29549671] (Human)
• JV Subiela, SH Torres, JB De Sanctis, N Hernández Cardiorespiratory responses, nitric oxide production and inflammatory factors in patients with myocardial infarction after rehabilitation Nitric Oxide, 2018-03-10;0(0):. 2018-03-10 [PMID: 29534920] (Human)

FAQs for eNOS (DY950-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NOS3