Human EGF DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human EGF DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: EGF

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human EGF DuoSet ELISA, 15 Plate

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Publications for EGF (DY236)(24)

Publications using DY236

• Tamar, C;Greenfield, K;McDonald, K;Levy, E;Brumbaugh, JE;Knoop, K; EGF and IgA in maternal milk, donor milk, and milk fortifiers in the neonatal intensive care unit setting PloS one 2025-04-16 [PMID: 40238773] (Human)
• Terczynska, M;Bargiel, W;Grabarczyk, M;Kozlowski, T;Zakowicz, P;Bojarski, D;Wasicka-Przewozna, K;Kapelski, P;Rajewska-Rager, A;Skibinska, M; Circulating Growth Factors and Cytokines Correlate with Temperament and Character Dimensions in Adolescents with Mood Disorders Brain sciences 2025-01-26 [PMID: 40002454] (Human)
• Delila, L;Nebie, O;Le, NTN;Timmerman, K;Lee, DY;Wu, YW;Chou, ML;Buée, L;Chou, SY;Blum, D;Devos, D;Burnouf, T; Neuroprotective effects of intranasal extracellular vesicles from human platelet concentrates supernatants in traumatic brain injury and Parkinson's disease models Journal of biomedical science 2024-09-05 [PMID: 39237980] (Human)
• T Deissova, M Cvanova, Z Kala, Z Jiraskova, J Dolina, L Kunovsky, R Kroupa, Z Pavlovsky, B Lipovy, Z Danek, L Izakovicov, O Urban, V Navratil, R Lischke, T Harustiak, T Grolich, V Prochazka, O Slaby, P Borilova L Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett's Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study Disease Markers, 2022-08-31;2022(0):8790748. 2022-08-31 [PMID: 36092955] (Human)
• K Zwittnig, B Kirnbauer, N Jakse, P Schlenke, I Mischak, S Ghanaati, S Al-Maawi, D Végh, M Payer, TA Zrnc Growth Factor Release within Liquid and Solid PRF Journal of Clinical Medicine, 2022-08-29;11(17):. 2022-08-29 [PMID: 36078998] (Human)
• S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis Journal of ovarian research, 2022-07-26;15(1):87. 2022-07-26 [PMID: 35883110] (Human)
• CW Lee, CC Kuo, CJ Liang, HJ Pan, CN Shen, CH Lee Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells Oncogene, 2022-07-06;23(1):26. 2022-07-06 [PMID: 35794526] (Human)
• ME Rhéaume, J Perreault, D Fournier, P Trépanier Preparation and growth factor characterization of cord blood-derived plasma, serum, growth factor-rich plasma and induced serum Cytokine, 2021-11-08;149(0):155756. 2021-11-08 [PMID: 34763157] (Human)
• XC Lo, JY Li, MT Lee, DJ Yao Frequency Shift of a SH-SAW Biosensor with Glutaraldehyde and 3-Aminopropyltriethoxysilane Functionalized Films for Detection of Epidermal Growth Factor Biosensors (Basel), 2020-08-05;10(8):. 2020-08-05 [PMID: 32764513] (Human)
• SJ Wang, R Li, TSC Ng, G Luthria, MJ Oudin, M Prytyskach, RH Kohler, R Weissleder, DA Lauffenbur, MA Miller Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy Sci Adv, 2020-05-22;6(21):eaaz8521. 2020-05-22 [PMID: 32494745] (Human)
• O Nebie, D Devos, V Vingtdeux, L Barro, JC Devedjian, A Jonneaux, ML Chou, R Bordet, L Buée, F Knutson, D Blum, T Burnouf The neuroprotective activity of heat-treated human platelet lysate biomaterials manufactured from outdated pathogen-reduced (amotosalen/UVA) platelet concentrates J. Biomed. Sci., 2019-10-31;26(1):89. 2019-10-31 [PMID: 31666073] (Human)
• A Sampera, FJ Sánchez-Ma, O Arpí, L Visa, M Iglesias, S Menéndez, É Gaye, A Dalmases, S Clavé, M Gelabert-B, TT Poulsen, M Kragh, B Bellosillo, J Albanell, A Rovira, C Montagut HER-family ligands promote acquired resistance to trastuzumab in gastric cancer Mol. Cancer Ther., 2019-09-04;0(0):. 2019-09-04 [PMID: 31484705] (Human)
• MS Chen, TJ Wang, HC Lin, B Thierry Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells New biotechnology, 2018-11-20;0(0):. 2018-11-20 [PMID: 30465908] (Human)
• L Gao, W Zhang, WQ Zhong, ZJ Liu, HM Li, ZL Yu, YF Zhao Tumor associated macrophages induce epithelial to mesenchymal transition via the EGFR/ERK1/2 pathway in head and neck squamous cell carcinoma Oncol. Rep., 2018-08-17;40(5):2558-2572. 2018-08-17 [PMID: 30132555]
• J Miku?a-Pie, P Uruski, S Szubert, K Maksin, R Moszy?ski, D Szpurek, A Wo?niak, S Sajdak, A Tykarski, K Ksi??ek The Proangiogenic Capabilities of Malignant Ascites Generated by Aggressive Ovarian Tumors Biomed Res Int, 2017-09-20;2017(0):2592496. 2017-09-20 [PMID: 29085834] (Human)
• Y Miura, Y Sukawa, S Hironaka, M Mori, K Nishikawa, S Tokunaga, H Okuda, T Sakamoto, K Taku, K Nishikawa, T Moriwaki, Y Negoro, Y Kimura, K Uchino, K Shinozaki, H Shinozaki, N Musha, H Yoshiyama, T Tsuda, Y Miyata, N Sugimoto, T Shirakawa, M Ito, K Yonesaka, K Yoshimura, N Boku, K Nosho, T Takano, I Hyodo Five-weekly S-1 plus cisplatin therapy combined with trastuzumab therapy in HER2-positive gastric cancer: a phase II trial and biomarker study (WJOG7212G) Gastric Cancer, 2017-05-11;0(0):. 2017-05-11 [PMID: 28497176] (Human)
• K El Bagdadi, A Kubesch, X Yu, S Al-Maawi, A Orlowska, A Dias, P Booms, E Dohle, R Sader, CJ Kirkpatric, J Choukroun, S Ghanaati Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept) Eur J Trauma Emerg Surg, 2017-03-21;0(0):. 2017-03-21 [PMID: 28324162] (Human)
• Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 2011-04-11;17(11):3619-30. 2011-04-11 [PMID: 21482691] (Human)
• Hickinson DM, Marshall GB, Beran GJ Identification of biomarkers in human head and neck tumor cell lines that predict for in vitro sensitivity to gefitinib. Clin Transl Sci, 2009-06-01;2(3):183-92. 2009-06-01 [PMID: 20443891] (Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (Human)
• Inoue Y, Miyamoto S, Fukami T, Shirota K, Yotsumoto F, Kawarabayashi T Amphiregulin is much more abundantly expressed than transforming growth factor-alpha and epidermal growth factor in human follicular fluid obtained from patients undergoing in vitro fertilization-embryo transfer. Fertil. Steril., 2008-03-05;91(4):1035-41. 2008-03-05 [PMID: 18325497] (Human)
• Yotsumoto F, Yagi H, Suzuki SO, Oki E, Tsujioka H, Hachisuga T, Sonoda K, Kawarabayashi T, Mekada E, Miyamoto S Validation of HB-EGF and amphiregulin as targets for human cancer therapy. Biochem. Biophys. Res. Commun., 2007-11-20;365(3):555-61. 2007-11-20 [PMID: 18023415] (Human)
• Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer. Cancer Cell, 2006-07-01;10(1):39-50. 2006-07-01 [PMID: 16843264] (Human)
• Sakai K, Yokote H, Murakami-Murofushi K, Tamura T, Saijo N, Nishio K In-frame deletion in the EGF receptor alters kinase inhibition by gefitinib. Biochem. J., 2006-08-01;397(3):537-43. 2006-08-01 [PMID: 16623663] (Human)

FAQs for EGF (DY236). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: EGF