Human Cytochrome c Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Cytochrome c Quantikine ELISA Kit Summary

• Background: The Quantikine Human Cytochrome c Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human cytochrome c in cell lysates.
• Specificity: Natural human Cytochrome c
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CYCS

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with other substances.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Cytochrome c Quantikine ELISA Kit

• CYCHCS
• CYCS
• Cytochrome c
• cytochrome c, somatic
• THC4

Background

Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Cytochrome c (DCTC0)(34)

Publications using DCTC0

• Liu, Q;Wu, L; Study on the regulation of trophoblast activity by abnormally expressed hsa_circ_0002768 in patients with gestational diabetes mellitus Ginekologia polska 2025-01-29 [PMID: 39878754] (Human)
• Quagliariello, V;Berretta, M;Bisceglia, I;Giacobbe, I;Iovine, M;Giordano, V;Arianna, R;Barbato, M;Izzo, F;Maurea, C;Canale, ML;Paccone, A;Inno, A;Scherillo, M;Gabrielli, D;Maurea, N; The sGCa Vericiguat Exhibit Cardioprotective and Anti-Sarcopenic Effects through NLRP-3 Pathways: Potential Benefits for Anthracycline-Treated Cancer Patients Cancers 2024-04-12 [PMID: 38672567] (Human)
• Q Chen, KO Nwozor, M van den Be, DJ Slebos, A Faiz, MR Jonker, HM Boezen, IH Heijink, M de Vries From Differential DNA Methylation in COPD to Mitochondria: Regulation of AHRR Expression Affects Airway Epithelial Response to Cigarette Smoke Cells, 2022-10-29;11(21):. 2022-10-29 [PMID: 36359818] (Human)
• MI Faizan, R Chaudhuri, S Sagar, S Albogami, N Chaudhary, I Azmi, A Akhtar, SM Ali, R Kumar, J Iqbal, MC Joshi, G Kharya, P Seth, SS Roy, T Ahmad NSP4 and ORF9b of SARS-CoV-2 Induce Pro-Inflammatory Mitochondrial DNA Release in Inner Membrane-Derived Vesicles Cells, 2022-09-23;11(19):. 2022-09-23 [PMID: 36230930] (Human)
• H Li, L Liu, Z Cao, W Li, R Liu, Y Chen, C Li, Y Song, G Liu, J Hu, Z Liu, C Lu, Y Liu Naringenin ameliorates homocysteine induced endothelial damage via the AMPKalpha/Sirt1 pathway Journal of advanced research, 2021-01-23;34(0):137-147. 2021-01-23 [PMID: 35024186] (Human)
• JH Lee, DH Ha, HK Go, J Youn, HK Kim, RC Jin, RB Miller, DH Kim, BS Cho, YW Yi Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury Int J Mol Sci, 2020-07-05;21(13):. 2020-07-05 [PMID: 32635660] (Human)
• C Tang, J Hou, G Yan, Y Qiao, D Wang, B Zhu, B Liu, E Luo, AQ Nawabi, L Chen Effects of Serum Cytochrome c on Contrast-Induced Nephropathy in Patients with ST-Elevation Myocardial Infarction Undergoing Percutaneous Coronary Intervention Biomed Res Int, 2019-01-23;2019(0):9357203. 2019-01-23 [PMID: 30809550] (Human)
• R De, S Sarkar, S Mazumder, S Debsharma, AA Siddiqui, SJ Saha, C Banerjee, S Nag, D Saha, S Pramanik, U Bandyopadh Macrophage migration inhibitory factor regulates mitochondrial dynamics and cell growth of human cancer cell lines through CD74-NF?B signaling J. Biol. Chem., 2018-10-26;0(0):. 2018-10-26 [PMID: 30366984] (Human)
• Ming Wu Anti-Cancer Effect of Cap-Translation Inhibitor 4EGI-1 in Human Glioma U87 Cells: Involvement of Mitochondrial Dysfunction and ER Stress Cell. Physiol. Biochem, 2016-12-12;40(5):1013-1028. 2016-12-12 [PMID: 27941351] (Human)
• Antonio L Bartorelli Diagnostic and Prognostic Utility of Circulating Cytochrome c in Acute Myocardial Infarction Circ. Res., 2016-10-31;0(0):. 2016-10-31 [PMID: 27799252] (Human)
• Chen Y, Chernyavsky A, Webber R, Grando S, Wang P Critical Role of the Neonatal Fc Receptor (FcRn) in the Pathogenic Action of Antimitochondrial Autoantibodies Synergizing with Anti-desmoglein Autoantibodies in Pemphigus Vulgaris. J Biol Chem, 2015-08-10;290(39):23826-37. 2015-08-10 [PMID: 26260795] (Human)
• Chernyavsky A, Shchepotin I, Galitovkiy V, Grando S Mechanisms of tumor-promoting activities of nicotine in lung cancer: synergistic effects of cell membrane and mitochondrial nicotinic acetylcholine receptors. BMC Cancer, 2015-03-19;15(0):152. 2015-03-19 [PMID: 25885699] (Human)
• Abdullah A, Mohammed A, Rasedee A, Mirghani M Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor positive breast cancer MCF-7 cells. Int J Mol Sci, 2015-02-05;16(2):3528-36. 2015-02-05 [PMID: 25664859] (Human)
• Sjovall F, Morota S, Asander Frostner E, Hansson M, Elmer E Cytokine and nitric oxide levels in patients with sepsis--temporal evolvement and relation to platelet mitochondrial respiratory function. PLoS ONE, 2014-05-14;9(5):e97673. 2014-05-14 [PMID: 24828117] (Human)
• Shin S, Jung E, Kim S, Kim J, Kim E, Lee J, Park D Antagonizing effects and mechanisms of afzelin against UVB-induced cell damage. PLoS ONE, 2013-04-23;8(4):e61971. 2013-04-23 [PMID: 23626759] (Human)
• Marchenko S, Chernyavsky AI, Arredondo J, Gindi V, Grando SA Antimitochondrial autoantibodies in pemphigus vulgaris: a missing link in disease pathophysiology. J. Biol. Chem., 2009-12-10;285(6):3695-704. 2009-12-10 [PMID: 20007702] (Human)
• Saliba J, Kasim NR, Tamboli RA, Isbell JM, Marks P, Feurer ID, Ikizler A, Abumrad NN Roux-en-Y gastric bypass reverses renal glomerular but not tubular abnormalities in excessively obese diabetics. Surgery, 2009-12-11;147(2):282-7. 2009-12-11 [PMID: 20004430] (Human)
• Dutta-Simmons J, Zhang Y, Gorgun G, Gatt M, Mani M, Hideshima T, Takada K, Carlson NE, Carrasco DE, Tai YT, Raje N, Letai AG, Anderson KC, Carrasco DR Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression. Blood, 2009-08-03;114(13):2699-708. 2009-08-03 [PMID: 19652203] (Human)
• De Milito A, Iessi E, Logozzi M, Lozupone F, Spada M, Marino ML, Federici C, Perdicchio M, Matarrese P, Lugini L, Nilsson A, Fais S Proton pump inhibitors induce apoptosis of human B-cell tumors through a caspase-independent mechanism involving reactive oxygen species. Cancer Res., 2007-06-01;67(11):5408-17. 2007-06-01 [PMID: 17545622] (Human)
• Li Y, Sagar MB, Wassler M, Shelat H, Geng YJ Apolipoprotein-J prevention of fetal cardiac myoblast apoptosis induced by ethanol. Biochem. Biophys. Res. Commun., 2007-03-28;357(1):157-61. 2007-03-28 [PMID: 17416353] (Canine)
• Lee CS, Kim YJ, Han ES Glycyrrhizin protection against 3-morpholinosydnonime-induced mitochondrial dysfunction and cell death in lung epithelial cells. Life Sci., 2007-02-11;80(19):1759-67. 2007-02-11 [PMID: 17346752] (Human)
• Mookherjee P, Quintanilla R, Roh MS, Zmijewska AA, Jope RS, Johnson GV Mitochondrial-targeted active Akt protects SH-SY5Y neuroblastoma cells from staurosporine-induced apoptotic cell death. J. Cell. Biochem., 2007-09-01;102(1):196-210. 2007-09-01 [PMID: 17340627] (Human)
• Kim H, Rafiuddin-Shah M, Tu HC, Jeffers JR, Zambetti GP, Hsieh JJ, Cheng EH Hierarchical regulation of mitochondrion-dependent apoptosis by BCL-2 subfamilies. Nat. Cell Biol., 2006-11-19;8(12):1348-58. 2006-11-19 [PMID: 17115033] (Human)
• Chen W, He FY, Li YQ The apoptosis effect of hispolon from Phellinus linteus (Berkeley & Curtis) Teng on human epidermoid KB cells. J Ethnopharmacol, 2006-02-28;105(1):280-5. 2006-02-28 [PMID: 16563677] (Human)
• Jow GM, Chou CJ, Chen BF, Tsai JH Beauvericin induces cytotoxic effects in human acute lymphoblastic leukemia cells through cytochrome c release, caspase 3 activation: the causative role of calcium. Cancer Lett., 2004-12-28;216(2):165-73. 2004-12-28 [PMID: 15533592] (Human)
• Bang JH, Han ES, Lim I Differential response of MG132 cytotoxicity against small cell lung cancer cells to changes in cellular GSH contents. Biochem. Pharmacol., 2004-08-15;68(4):659-66. 2004-08-15 [PMID: 15276073] (Human)
• Ali N, Yoshizumi M, Tsuchiya K, Kyaw M, Fujita Y, Izawa Y, Abe S, Kanematsu Y, Kagami S, Tamaki T Ebselen inhibits p38 mitogen-activated protein kinase-mediated endothelial cell death by hydrogen peroxide. Eur. J. Pharmacol., 2004-02-06;485(1):127-35. 2004-02-06 [PMID: 14757132] (Human)
• Panduri V, 107352, Weitzman SA, Chandel N, Kamp DW The mitochondria-regulated death pathway mediates asbestos-induced alveolar epithelial cell apoptosis. Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells, 2003-02-01;28(2):241-8. 2003-02-01 [PMID: 12540492] (Human)
• Brichese L, Barboule N, Heliez C, Valette A Bcl-2 phosphorylation and proteasome-dependent degradation induced by paclitaxel treatment: consequences on sensitivity of isolated mitochondria to Bid. Exp. Cell Res., 2002-08-01;278(1):101-11. 2002-08-01 [PMID: 12126962] (Human)
• Jemmerson R, LaPlante B, Treeful A Release of intact, monomeric cytochrome c from apoptotic and necrotic cells. Cell Death Differ., 2002-05-01;9(5):538-48. 2002-05-01 [PMID: 11973612] (Human)
• Rajalingam K, Al-Younes H, Muller A, Meyer TF, Szczepek AJ, Rudel T Epithelial cells infected with Chlamydophila pneumoniae (Chlamydia pneumoniae) are resistant to apoptosis. Infect. Immun., 2001-12-01;69(12):7880-8. 2001-12-01 [PMID: 11705971] (Human)
• Poulaki V, Mitsiades N, Romero ME, Tsokos M Fas-mediated apoptosis in neuroblastoma requires mitochondrial activation and is inhibited by FLICE inhibitor protein and Bcl-2. Cancer Res., 2001-06-15;61(12):4864-72. 2001-06-15 [PMID: 11406564] (Human)
• Jiang C, Wang Z, Ganther H, Lu J Caspases as key executors of methyl selenium-induced apoptosis (anoikis) of DU-145 prostate cancer cells. Cancer Res., 2001-04-01;61(7):3062-70. 2001-04-01 [PMID: 11306488] (Human)

Bioinformatics

• Gene Symbol: CYCS