Human CD40 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CD40 Quantikine ELISA Kit Summary

• Background: The Quantikine Human CD40 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CD40 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human CD40 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD40 showed linear curves that were parallel to the standard curv...es obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD40.
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• Specificity: Natural and recombinant human CD40
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CD40

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD40 Quantikine ELISA Kit

• B cell surface antigen CD40
• B-cell surface antigen CD40
• Bp50B cell-associated molecule
• CD40 antigen
• CD40 molecule, TNF receptor superfamily member 5
• CD40 type II isoform
• CD40
• CD40L receptor
• CDw40
• MGC9013
• nerve growth factor receptor-related B-lymphocyte activation molecule
• p50
• TNFRSF5
• TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
• tumor necrosis factor receptor superfamily member 5
• tumor necrosis factor receptor superfamily, member 5

Background

CD40, also known as TNFRSF5, is a transmembrane TNF superfamily receptor that is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. Interaction of CD40 with CD40 Ligand leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells. This interaction promotes B cell activation and T cell-dependent humoral responses. CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy. Dysregulation of CD40/CD40 Ligand expression and interactions contributes to disease severity in AIDS, atherosclerosis, atherothrombosis, and restenosis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CD40/TNFRSF5 (DCCD40)(6)

Publications using DCCD40

• David, P;Kouhestani, D;Hansen, FJ;Paul, S;Czubayko, F;Karabiber, A;Weisel, N;Klösch, B;Merkel, S;Ole-Baur, J;Gie beta l, A;Van Deun, J;Vera, J;Mittelstädt, A;Weber, GF; Exosomal CD40, CD25, and Serum CA19-9 as Combinatory Novel Liquid Biopsy Biomarker for the Diagnosis and Prognosis of Patients with Pancreatic Ductal Adenocarcinoma International journal of molecular sciences 2025-02-11 [PMID: 40003965] (Human)
• Klersy, A;Meyer, S;Leuschner, F;Kessler, T;Hecker, M;Wagner, AH; Ectodomain Shedding by ADAM17 Increases the Release of Soluble CD40 from Human Endothelial Cells under Pro-Inflammatory Conditions Cells 2023-07-25 [PMID: 37566005] (Human)
• Z Albar, M Albakri, J Hajjari, M Karnib, SE Janus, SG Al-Kindi Inflammatory Markers and Risk of Heart Failure With Reduced to Preserved Ejection Fraction The American journal of cardiology, 2022-01-03;0(0):. 2022-01-03 [PMID: 34986991] (Human)
• S Meltzer, A Torgunrud, H Abrahamsso, AM Solbakken, K Flatmark, S Dueland, KM Bakke, PA Bousquet, A Negård, C Johansen, LG Lyckander, FO Larsen, JV Schou, KR Redalen, AH Ree The circulating soluble form of the CD40 costimulatory immune checkpoint receptor and liver metastasis risk in rectal cancer British Journal of Cancer, 2021-04-09;0(0):. 2021-04-09 [PMID: 33837301] (Human)
• K Lashkari, GC Teague, U Beattie, J Betts, S Kumar, MM McLaughlin, FJ López Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration PLoS ONE, 2020-08-07;15(8):e0236283. 2020-08-07 [PMID: 32764794] (Human)
• DE Martínez-F, JR Padilla-Gu, F Casillas-M, E Valdés-Alv, B Parra-Reyn, M Aceves-Ram, JF Muñoz-Vall, U Zalapa Flo, JC Chávez Her, Y Valle Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome J Immunol Res, 2019-04-30;2019(0):8063983. 2019-04-30 [PMID: 31183392] (Human)

FAQs for CD40/TNFRSF5 (DCCD40). (Showing 1 - 3 of 3 FAQs).

1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
   • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
   • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.
3. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CD40