Human CD163 DuoSet ELISA, 15 Plate

Correlation between European League Against Rheumatism Sjögren’s Syndrome Disease Activity Index (ESSDAI) scores and concentrations of nine screened serum proteins analyzed by ELISA in the validation cohort of patients with primary Sjögren’s syndrome (n = 58). The nine proteins were CXCL13, TNF receptor 2 (TNF-R2), CD48, B-cell activating factor (BAFF), programmed cell death protein 1 ligand 2 (PD-L2), lymphocyte activation gene-3 (LAG-3), beta 2-microglobulin ( beta 2MG), ephrin type-B receptor 2 (EPHB2), and CD163. Pearson’s correlation test was used. The correlation coefficients (r) and P values are shown in the table. P <0.05 was considered significant Image collected and cropped by CiteAb from the following open publication (//arthritis-research.biomedcentral.com/articles/10.1186/s13075-016-1006-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CD163 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CD163

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD163 DuoSet ELISA, 15 Plate

• CD_antigen: CD163
• CD163 molecule
• CD163
• GHI/61
• HbSR
• Hemoglobin scavenger receptor
• M130
• macrophage-associated antigen
• MM130
• RM3/1
• SCARI1
• scavenger receptor cysteine-rich type 1 protein M130
• sCD163
• Soluble CD163

Background

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Publications for CD163 (DY1607)(32)

Publications using DY1607

• Dos Santos, CF;Nunes, PCG;Fiestas-Solorzano, VE;Gandini, M;Dos Santos, FB;Pinheiro, RO;de Souza, LJ;Damasco, PV;de Oliveira Pinto, LM;de Azeredo, EL; Monocyte Dynamics in Chikungunya Fever: Sustained Activation and Vascular-Coagulation Pathway Involvement Viruses 2025-09-07 [PMID: 41012652] (Human)
• Schneider, FM;Pochert, N;Schittek, F;Köpke, MB;Wild, CM;Veh, JM;Rohrmoser, NR;Kuhn, C;Urban, B;Kahl, KH;Schneider, M;Mattmer, A;Hinske, LC;Fluhrer, R;Golas, MM;Untch, M;Kuehn, T;Banys-Paluchowski, M;Jeschke, U;Traidl-Hoffmann, C;Dannecker, C;Ditsch, N; Macrophage polarization is associated with postoperative seroma development in breast cancer in the SerMa pilot cohort Scientific reports 2025-09-12 [PMID: 40940394] (Human)
• Gkiokas, A;Papadatou-Gigante, M;Gkioka, AI;Koudouna, A;Tryfou, TM;Alexandropoulos, A;Bartzi, V;Kafasi, N;Kyrtsonis, MC; Tumor-Associated Macrophage (TAM)-Related Cytokines, sCD163, CCL2, and CCL4, as Novel Biomarkers for Overall Survival and Time to Treatment in Waldenstrom's Macroglobulinemia: Emphasis on Asymptomatic WM Cells 2025-02-13 [PMID: 39996747] (Human)
• Williams, ME;Asia, LK;Lindeque, Z;Jansen van Vuren, E; The association between HIV-1 Tat and Vif amino acid sequence variation, inflammation and Trp-Kyn metabolism: an exploratory investigation BMC infectious diseases 2024-09-09 [PMID: 39251983] (Human)
• Grannes, H;Ueland, T;Simeone, P;Liani, R;Guagnano, MT;Aukrust, P;Michelsen, AE;Birkeland, K;di Castelnuovo, A;Cipollone, F;Consoli, A;Halvorsen, B;Gregersen, I;Santilli, F; Liraglutide and not lifestyle intervention reduces soluble CD163 after comparable weight loss in obese participants with prediabetes or type 2 diabetes mellitus Cardiovascular diabetology 2024-04-29 [PMID: 38685051] (Human)
• Evans, C;Mutasa, K;Rukobo, S;Govha, M;Mushayanembwa, P;Chasekwa, B;Majo, FD;Tavengwa, NV;Broad, J;Noble, C;Gough, EK;Kelly, P;Bourke, CD;Humphrey, JH;Ntozini, R;Prendergast, AJ; Inflammation and cytomegalovirus viremia during pregnancy drive sex-differentiated differences in mortality and immune development in HIV-exposed infants Nature communications 2024-04-17 [PMID: 38632279] (Human)
• Giron, LB;Liu, Q;Adeniji, OS;Yin, X;Kannan, T;Ding, J;Lu, DY;Langan, S;Zhang, J;Azevedo, JLLC;Li, SH;Shalygin, S;Azadi, P;Hanna, DB;Ofotokun, I;Lazar, J;Fischl, MA;Haberlen, S;Macatangay, B;Adimora, AA;Jamieson, BD;Rinaldo, C;Merenstein, D;Roan, NR;Kutsch, O;Gange, S;Wolinsky, SM;Witt, MD;Post, WS;Kossenkov, A;Landay, AL;Frank, I;Tien, PC;Gross, R;Brown, TT;Abdel-Mohsen, M; Immunoglobulin G N-glycan markers of accelerated biological aging during chronic HIV infection Nature communications 2024-04-10 [PMID: 38600088] (Human)
• Li, JZ;Melberg, M;Kittilson, A;Abdel-Mohsen, M;Li, Y;Aga, E;Bosch, RJ;Wonderlich, ER;Kinslow, J;Giron, LB;Di Germanio, C;Pilkinton, M;MacLaren, L;Keefer, M;Fox, L;Barr, L;Acosta, E;Ananworanich, J;Coombs, R;Mellors, J;Deeks, S;Gandhi, RT;Busch, M;Landay, A;Macatangay, B;Smith, DM;AIDS Clinical Trials Group A5345 Study Team, ; Predictors of HIV rebound differ by timing of antiretroviral therapy initiation JCI insight 2024-02-08 [PMID: 38329130] (Human)
• Giron, LB;Liu, Q;Adeniji, OS;Yin, X;Kannan, T;Ding, J;Lu, DY;Langan, S;Zhang, J;Azevedo, JLLC;Hanna, DB;Ofotokun, I;Lazar, J;Fischl, MA;Haberlen, S;Macatangay, B;Adimora, AA;Jamieson, BD;Rinaldo, C;Merenstein, D;Roan, NR;Kutsch, O;Gange, S;Wolinsky, S;Witt, M;Post, WS;Kossenkov, A;Landay, A;Frank, I;Tien, PC;Gross, R;Brown, TT;Abdel-Mohsen, M; Plasma Glycomic Markers of Accelerated Biological Aging During Chronic HIV Infection bioRxiv : the preprint server for biology 2023-08-10 [PMID: 37609144] (ELISA, Human)
• M Ackermann, JC Kamp, C Werlein, CL Walsh, H Stark, V Prade, R Surabattul, WL Wagner, C Disney, AJ Bodey, T Illig, DJ Leeming, MA Karsdal, A Tzankov, P Boor, MP Kühnel, FP Länger, SE Verleden, HM Kvasnicka, HH Kreipe, A Haverich, SM Black, A Walch, P Tafforeau, PD Lee, MM Hoeper, T Welte, B Seeliger, S David, D Schuppan, SJ Mentzer, DD Jonigk The fatal trajectory of pulmonary COVID-19 is driven by lobular ischemia and fibrotic remodelling EBioMedicine, 2022-10-04;85(0):104296. 2022-10-04 [PMID: 36206625] (Human)
• H Kared, AS Wolf, A Alirezayla, A Ravussin, G Solum, TT Tran, F Lund-Johan, JT Vaage, LS Nissen-Mey, UC Nygaard, O Hungnes, AH Robertson, LM Næss, L Trogstad, P Magnus, LA Munthe, S Mjaaland Immune responses in Omicron SARS-CoV-2 breakthrough infection in vaccinated adults Nature Communications, 2022-07-18;13(1):4165. 2022-07-18 [PMID: 35851055] (Human)
• V Cairoli, E De Matteo, P Casciato, B Ameigeiras, MV Preciado, P Valva The performance of soluble CD163 as a non-invasive biomarker of liver damage in chronically HCV and HCV/HIV infected subjects PLoS ONE, 2022-07-07;17(7):e0270911. 2022-07-07 [PMID: 35797388] (Human)
• RB da Silva P, MA Mendes, VC Soares, J França-Cos, AM Sales, NC Duppré, V de Matos B, TP da Silva, PT Bozza, MT Bozza, EN Sarno, MO Moraes, GM Sperandio, RO Pinheiro Arginase 1 is a marker of protection against illness in contacts of leprosy patients Scientific Reports, 2022-05-12;12(1):7850. 2022-05-12 [PMID: 35552484] (Human)
• H Maeno, PF Wong, S AbuBakar, M Yang, SS Sam, J Jamil-Abd, A Shunmugara, M Mustafa, RM Said, E Mageswaren, A Azmel, A Mat Jelani A 3D Microfluidic ELISA for the Detection of Severe Dengue: Sensitivity Improvement and Vroman Effect Amelioration by EDC-NHS Surface Modification Micromachines, 2021-11-30;12(12):. 2021-11-30 [PMID: 34945351] (Human)
• C Kelly, W Tinago, D Alber, P Hunter, N Luckhurst, J Connolly, F Arrigoni, A Garcia Abn, R Kamn'gona, I Sheha, M Chammudzi, K Jambo, J Mallewa, A Rapala, PWG Mallon, H Mwandumba, N Klein, S Khoo Inflammatory pathways amongst people living with HIV in Malawi differ according to socioeconomic status PLoS ONE, 2021-08-25;16(8):e0256576. 2021-08-25 [PMID: 34432828] (Human)
• HK Pihlstrøm, T Ueland, AE Michelsen, P Aukrust, F Gatti, C Hammarströ, M Kasprzycka, J Wang, G Haraldsen, G Mjøen, DO Dahle, K Midtvedt, IA Eide, A Hartmann, H Holdaas Exploring the potential effect of paricalcitol on markers of inflammation in de novo renal transplant recipients PLoS ONE, 2020-12-16;15(12):e0243759. 2020-12-16 [PMID: 33326471] (Human)
• S Novelli, C Lécuroux, C Goujard, J Reynes, A Villemant, L Blum, A Essat, V Avettand-F, O Launay, JM Molina, C Bourgeois, L Meyer Persistence of monocyte activation under treatment in people followed since acute HIV-1 infection relative to participants at high or low risk of HIV infection EBioMedicine, 2020-11-26;62(0):103129. 2020-11-26 [PMID: 33248370] (Human)
• I Vujkovic-C, O Sortino, E Verheij, J Sklar, FW Wit, NA Kootstra, B Sellers, JM Brenchley, J Ananworani, MSV Loeff, Y Belkaid, P Reiss, I Sereti HIV-associated gut dysbiosis is independent of sexual practice and correlates with noncommunicable diseases Nat Commun, 2020-05-15;11(1):2448. 2020-05-15 [PMID: 32415070] (Human)
• X Ruiz-Plaza, E Rodríguez-, M Alves, A Altuna-Coy, J Lozano-Bar, M Portero-Ot, JF García-Fon, S Martínez-G, J Segarra, MR Chacón Biofluid quantification of TWEAK/Fn14 axis in combination with a selected biomarker panel improves assessment of prostate cancer aggressiveness J Transl Med, 2019-09-09;17(1):307. 2019-09-09 [PMID: 31500625] (Human)
• J Dereke, J Nilsson, C Nilsson, H Strevens, M Landin-Ols, M Hillman Soluble CD163 and TWEAK in early pregnancy gestational diabetes and later glucose intolerance PLoS ONE, 2019-05-09;14(5):e0216728. 2019-05-09 [PMID: 31071174] (Human)
• GA Gualdoni, GA Hofmann, P Wohlfarth, HM Winkler, S Winkler, H Haslacher, R Thalhammer, A Makristath, F Ratzinger, H Burgmann Prevalence and Outcome of Secondary Hemophagocytic Lymphohistiocytosis Among SIRS Patients: Results from a Prospective Cohort Study J Clin Med, 2019-04-19;8(4):. 2019-04-19 [PMID: 31010216] (Human)
• SS Foo, W Chen, Y Chan, WS Lee, SA Lee, G Cheng, K Nielsen-Sa, P Brasil, JU Jung Biomarkers and immunoprofiles associated with fetal abnormalities of ZIKV-positive pregnancies JCI Insight, 2018-11-02;3(21):. 2018-11-02 [PMID: 30385728] (Human)
• MS Espíndola, LS Soares, LJ Galvão-Lim, FA Zambuzi, MC Cacemiro, VS Brauer, CM Marzocchi-, M de Souza G, LR Amaral, OA Martins-Fi, VR Bollela, FG Frantz Epigenetic alterations are associated with monocyte immune dysfunctions in HIV-1 infection Sci Rep, 2018-04-03;8(1):5505. 2018-04-03 [PMID: 29615725] (Human)
• GJ Dekkema, WH Abdulahad, T Bijma, SM Moran, L Ryan, MA Little, CA Stegeman, P Heeringa, JF Sanders Urinary and serum soluble CD25 complements urinary soluble CD163 to detect active renal anti-neutrophil cytoplasmic autoantibody-associated vasculitis: a cohort study Nephrol. Dial. Transplant., 2019-02-01;0(0):. 2019-02-01 [PMID: 29506265] (Human)
• A Chéret, C Durier, A Mélard, M Ploquin, J Heitzmann, C Lécuroux, V Avettand-F, L David, G Pialoux, JM Chennebaul, M Müller-Tru, C Goujard, C Rouzioux, L Meyer Impact of early cART on HIV blood and semen compartments at the time of primary infection PLoS ONE, 2017-07-14;12(7):e0180191. 2017-07-14 [PMID: 28708873] (Human)
• T Booiman, FW Wit, I Maurer, D De Frances, CA Sabin, AM Harskamp, M Prins, P Garagnani, C Pirazzini, C Franceschi, D Fuchs, M Gisslén, A Winston, P Reiss, NA Kootstra High Cellular Monocyte Activation in People Living With Human Immunodeficiency Virus on Combination Antiretroviral Therapy and Lifestyle-Matched Controls Is Associated With Greater Inflammation in Cerebrospinal Fluid Open Forum Infect Dis, 2017-05-25;4(3):ofx108. 2017-05-25 [PMID: 28680905] (Human)
• T Ueland, LE Laugsand, LJ Vatten, I Janszky, C Platou, AE Michelsen, JK Damås, P Aukrust, BO Åsvold Monocyte/macrophage and T cell activation markers are not independently associated with MI risk in healthy individuals - results from the HUNT Study Int. J. Cardiol., 2017-06-12;0(0):. 2017-06-12 [PMID: 28615143] (Human)
• M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study Diabetol Metab Syndr, 2017-05-25;9(0):41. 2017-05-25 [PMID: 28559931] (Human)
• Identification of promising plasma immune biomarkers to differentiate active pulmonary tuberculosis Cytokine, 2016-08-31;88(0):99-107. 2016-08-31 [PMID: 27591510] (Human)
• Elevated Basal Pre-infection CXCL10 in Plasma and in the Small Intestine after Infection Are Associated with More Rapid HIV/SIV Disease Onset PLoS Pathog, 2016-08-10;12(8):e1005774. 2016-08-10 [PMID: 27509048] (Human)
• Gram M, Anderson U, Johansson M, Edstrom-Hagerwall A, Larsson I, Jalmby M, Hansson S, Akerstrom B The Human Endogenous Protection System against Cell-Free Hemoglobin and Heme Is Overwhelmed in Preeclampsia and Provides Potential Biomarkers and Clinical Indicators. PLoS ONE, 2015-09-14;10(9):e0138111. 2015-09-14 [PMID: 26368565] (Human)
• Rojo-Martinez G, Maymo-Masip E, Rodriguez M, Solano E, Goday A, Soriguer F, Valdes S, Chaves F, Delgado E, Colomo N, Hernandez P, Vendrell J, Chacon M Serum sCD163 levels are associated with type 2 diabetes mellitus and are influenced by coffee and wine consumption: results of the Di@bet.es study. PLoS ONE, 2014-06-30;9(6):e101250. 2014-06-30 [PMID: 24978196] (Human)

FAQs for CD163 (DY1607). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CD163