Human CCL3/MIP-1 alpha DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CCL3/MIP-1 alpha DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL3/MIP-1 alpha DuoSet ELISA, 15 Plate

• C-C motif chemokine 3
• MIP1-(a)
• AI323804
• CCL3
• chemokine (C-C motif) ligand 3
• G0S19-1
• LD78a
• LD78alpha
• MIP1 alpha
• MIP-1 alpha
• MIP1A
• MIP-1alpha
• MIP1-alpha
• PAT 464.1
• SCYA3
• SIS-beta

Background

MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.

The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.

Publications for CCL3/MIP-1 alpha (DY270)(56)

Publications using DY270

• Navas, A;Matzaraki, V;van Eekeren, LE;Blaauw, MJT;Groenendijk, AL;Vos, WAJW;Jacobs-Cleophas, M;Dos Santos, JC;van der Ven, AJAM;Joosten, LAB;Netea, MG; Plasma Proteomic Signature as a Predictor of Age Advancement in People Living With HIV Aging cell 2025-01-15 [PMID: 39812213] (Human)
• Rajamanickam, V;Desouza, CV;Castillo, RT;Saraswathi, V; Blocking Thromboxane-Prostanoid Receptor Signaling Attenuates Lipopolysaccharide- and Stearic Acid-Induced Inflammatory Response in Human PBMCs Cells 2024-08-08 [PMID: 39195211] (Human)
• Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M; Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls PloS one 2024-07-15 [PMID: 39008499] (Human)
• Marinho, A;Seabra, CL;Lima, SAC;Lobo-da-Cunha, A;Reis, S;Nunes, C; Empowering Naringin's Anti-Inflammatory Effects through Nanoencapsulation International journal of molecular sciences 2024-04-09 [PMID: 38673736] (Human)
• Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS; Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses Biomedicines 2024-03-25 [PMID: 38672079] (Human)
• Kim, YT;Huh, JW;Choi, YH;Yoon, HK;Nguyen, TT;Chun, E;Jeong, G;Park, S;Ahn, S;Lee, WK;Noh, YW;Lee, KS;Ahn, HS;Lee, C;Lee, SM;Kim, KS;Suh, GJ;Jeon, K;Kim, S;Jin, M; Highly secreted tryptophanyl tRNA synthetase 1 as a potential theranostic target for hypercytokinemic severe sepsis EMBO molecular medicine 2024-01-01 [PMID: 38177528] (Mouse)
• Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D; Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial PloS one 2023-11-02 [PMID: 37917760] (Human)
• Y Xie, A Chupina Es, B Nelson, H Feng, C Pothoulaki, L Chesnel, HW Koon ADS024, a Bacillus velezensis strain, protects human colonic epithelial cells against C. difficile toxin-mediated apoptosis Frontiers in Microbiology, 2023-01-10;13(0):1072534. 2023-01-10 [PMID: 36704560] (Human)
• TTT Nguyen, YH Choi, WK Lee, Y Ji, E Chun, YH Kim, JE Lee, HS Jung, JH Suh, S Kim, M Jin Tryptophan-dependent and -independent secretions of tryptophanyl- tRNA synthetase mediate innate inflammatory responses Cell Reports, 2022-12-30;42(1):111905. 2022-12-30 [PMID: 36640342] (Human)
• FE Rossi, AJ Maldonado, JM Cholewa, SLG Ribeiro, CA de Araújo, C Figueiredo, T Reichel, K Krüger, FS Lira, LG Minuzzi Exercise training-induced changes in immunometabolic markers in youth badminton athletes Scientific Reports, 2022-09-15;12(1):15539. 2022-09-15 [PMID: 36109571] (Human)
• MJ Tosiek, K Groesser, A Pekcec, M Zwirek, G Murugesan, E Borges Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response Journal of Immunology Research, 2022-02-25;2022(0):9926305. 2022-02-25 [PMID: 35252461] (Human)
• S Jamieson, A Mawdesley, D Deehan, J Kirby, J Holland, A Tyson-Capp Inflammatory responses to metal oxide ceramic nanopowders Scientific Reports, 2021-05-18;11(1):10531. 2021-05-18 [PMID: 34006936] (Human)
• R da Silva A, F Soldevila, M Pomaznoy, M Babor, J Bennett, Y Tian, NN Khalil, Y Qian, A Mandava, RH Scheuerman, M Cortese, B Pulendran, CD Petro, AP Gilkes, LA Purcell, A Sette, B Peters Systems view of Bordetella pertussis booster vaccination in adults primed with whole-cell vs. acellular vaccine JCI Insight, 2021-04-08;0(0):. 2021-04-08 [PMID: 33690224] (Human)
• GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation Biomolecules, 2021-02-21;11(2):. 2021-02-21 [PMID: 33669910] (Human)
• H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist Frontiers in Pharmacology, 2020-12-08;11(0):598702. 2020-12-08 [PMID: 33363471] (Human)
• E Yeregui, C Viladés, P Domingo, A Ceausu, YM Pacheco, S Veloso, A Inciarte, J Vidal-Gonz, M Peraire, C Perpiñán, V Falcó, J Masip, V Alba, M Vargas, A Martí, L Reverté, J Mallolas, F Vidal, J Peraire, A Rull High circulating SDF-1and MCP-1 levels and genetic variations in CXCL12, CCL2 and CCR5: Prognostic signature of immune recovery status in treated HIV-positive patients EBioMedicine, 2020-11-06;62(0):103077. 2020-11-06 [PMID: 33166788] (Human)
• B Wang, GL Bellot, K Iskandar, TW Chong, FY Goh, JJ Tai, H Schwarz, SC Wong, S Pervaiz Resveratrol attenuates TLR-4 mediated inflammation and elicits therapeutic potential in models of sepsis Sci Rep, 2020-11-02;10(1):18837. 2020-11-02 [PMID: 33139717] (Human)
• T Peeters, S Martens, V D'Onofrio, MHT Stappers, JCH van der Hi, B Houben, R Achten, LAB Joosten, IC Gyssens An observational study of innate immune responses in patients with acute appendicitis Sci Rep, 2020-10-15;10(1):17352. 2020-10-15 [PMID: 33060696] (Human)
• S Kochumon, H Arefanian, R Azim, S Shenouda, T Jacob, N Abu Khalaf, F Al-Rashed, A Hasan, S Sindhu, F Al-Mulla, R Ahmad Stearic Acid and TNF-&alpha Co-Operatively Potentiate MIP-1&alpha Production in Monocytic Cells via MyD88 Independent TLR4/TBK/IRF3 Signaling Pathway Biomedicines, 2020-10-09;8(10):. 2020-10-09 [PMID: 33050324] (Human)
• S Sindhu, N Akhter, A Wilson, R Thomas, H Arefanian, A Al Madhoun, F Al-Mulla, R Ahmad MIP-1alpha Expression Induced by Co-Stimulation of Human Monocytic Cells with Palmitate and TNF-alpha Involves the TLR4-IRF3 Pathway and Is Amplified by Oxidative Stress Cells, 2020-07-29;9(8):. 2020-07-29 [PMID: 32751118] (Human)
• S Abouelasra, M De Bondt, N Berghmans, M Gouwy, VLS de Oliveir, SC Oliveira, FA Amaral, P Proost, J Van Damme, S Struyf, M De Buck Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case Mediators Inflamm., 2020-07-06;2020(0):6087109. 2020-07-06 [PMID: 32694927] (Human)
• B Sadowska, J Rywaniak, A Cichocka, K Cichocka, J ?uchowski, U Wójcik-Boj, M Wi?ckowska, B Ró?alska Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnusrhamnoides (L.) A. Nelson-The Implications for Human Barrier Cells Molecules, 2020-05-09;25(9):. 2020-05-09 [PMID: 32397559] (Human)
• P Dimou, RD Wright, KL Budge, A Midgley, SC Satchell, M Peak, MW Beresford The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis Sci Rep, 2019-06-06;9(1):8348. 2019-06-06 [PMID: 31171837] (Human)
• G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5 Cell Death Dis, 2019-03-19;10(4):264. 2019-03-19 [PMID: 30890699] (Human)
• H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine Transl Oncol, 2018-12-08;12(3):397-403. 2018-12-08 [PMID: 30530187] (Human)
• Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL J. Cell. Mol. Med., 2018-10-24;0(0):. 2018-10-24 [PMID: 30353642] (Human)
• MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads PLoS ONE, 2018-01-25;13(1):e0191676. 2018-01-25 [PMID: 29370247] (Human)
• JM da Silva, TP Moreira Do, LM Sobral, CM Queiroz-Ju, MA Rachid, AEI Proudfoot, GP Garlet, AC Batista, MM Teixeira, AM Leopoldino, RC Russo, TA Silva Relevance of CCL3/CCR5 axis in oral carcinogenesis Oncotarget, 2017-04-06;8(31):51024-51036. 2017-04-06 [PMID: 28881626] (Human)
• J Venturini, RS Cavalcante, DV Moris, M de Assis G, AD Levorato, KH Dos Reis, MSP de Arruda, RP Mendes Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis Acta Trop., 2017-06-09;0(0):. 2017-06-09 [PMID: 28606816] (Human)
• Daniela Micheline Dos Santos Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in�vitro analysis J Prosthet Dent, 2016-11-09;0(0):. 2016-11-09 [PMID: 27836141]
• CD84 mediates CLL-microenvironment interactions Oncogene, 2016-07-25;0(0):. 2016-07-25 [PMID: 27452524] (Human)
• Harbort C, Soeiro-Pereira P, von Bernuth H, Kaindl A, Costa-Carvalho B, Condino-Neto A, Reichenbach J, Roesler J, Zychlinsky A, Amulic B Neutrophil oxidative burst activates ATM to regulate cytokine production and apoptosis. Blood, 2015-10-21;126(26):2842-51. 2015-10-21 [PMID: 26491069] (Human)
• Garcia-Ruiz G, Flores-Espinosa P, Preciado-Martinez E, Bermejo-Martinez L, Espejel-Nunez A, Estrada-Gutierrez G, Maida-Claros R, Flores-Pliego A, Zaga-Clavellina V In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1beta, TNFalpha, IL-6, IL-8, IL-10, MIP-1alpha, and MMP-9 in pre-labor human term placenta. Reprod Biol Endocrinol, 2015-10-07;13(0):115. 2015-10-07 [PMID: 26446923] (Human)
• Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand. Nat Commun, 2014-06-09;5(0):4092. 2014-06-09 [PMID: 24910129] (Human)
• Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro. PLoS ONE, 2014-05-12;9(5):e96925. 2014-05-12 [PMID: 24819779] (Human)
• Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway. PLoS ONE, 2013-12-30;8(12):e82867. 2013-12-30 [PMID: 24386121] (Human)
• Yamaguchi Y, Shao Z, Sharif S, Du X, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung L Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage. J Biol Chem, 2012-11-30;288(5):3097-111. 2012-11-30 [PMID: 23204518] (Human)
• Neuenschwander LC, Bittencourt H, Ribeiro AF, Teixeira AL, Teixeira MM, Teixeira JC, Nobre V Plasma levels of procalcitonin and eight additional inflammatory molecules in febrile neutropenic patients. Clinics (Sao Paulo), 2011-01-01;66(10):1699-705. 2011-01-01 [PMID: 22012040] (Human)
• Brietzke E, Kauer-Sant'Anna M, Teixeira AL, Kapczinski F Abnormalities in serum chemokine levels in euthymic patients with bipolar disorder. Brain Behav. Immun., 2009-05-04;23(8):1079-82. 2009-05-04 [PMID: 19406226] (Human)
• Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Mitsiades C, Chauhan D, Okawa Y, Munshi NC, Richardson PG, Anderson KC Lenalidomide inhibits osteoclastogenesis, survival factors and bone-remodeling markers in multiple myeloma. Leukemia, 2008-07-03;22(10):1925-32. 2008-07-03 [PMID: 18596740] (Human)
• Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation. Am. J. Transplant., 2008-07-01;8(7):1512-22. 2008-07-01 [PMID: 18513272] (Human)
• Raje N, Woo SB, Hande K, Yap JT, Richardson PG, Vallet S, Treister N, Hideshima T, Sheehy N, Chhetri S, Connell B, Xie W, Tai YT, Szot-Barnes A, Tian M, Schlossman RL, Weller E, Munshi NC, Van Den Abbeele AD, Anderson KC Clinical, radiographic, and biochemical characterization of multiple myeloma patients with osteonecrosis of the jaw. Clin. Cancer Res., 2008-04-15;14(8):2387-95. 2008-04-15 [PMID: 18413829] (Human)
• Ishitsuka K, Hideshima T, Neri P, Vallet S, Shiraishi N, Okawa Y, Shen Z, Raje N, Kiziltepe T, Ocio EM, Chauhan D, Tassone P, Munshi N, Campbell RM, Dios AD, Shih C, Starling JJ, Tamura K, Anderson KC p38 mitogen-activated protein kinase inhibitor LY2228820 enhances bortezomib-induced cytotoxicity and inhibits osteoclastogenesis in multiple myeloma; therapeutic implications. Br. J. Haematol., 2008-04-07;141(5):598-606. 2008-04-07 [PMID: 18397345] (Human)
• Li K, Dai D, Yao L, Gu X, Luan K, Tian W, Zhao Y, Wang B Association between the macrophage inflammatory protein-l alpha gene polymorphism and Alzheimer's disease in the Chinese population. Neurosci. Lett., 2008-01-10;433(2):125-8. 2008-01-10 [PMID: 18242850] (Human)
• Teixeira AL, Reis HJ, Nicolato R, Brito-Melo G, Correa H, Teixeira MM, Romano-Silva MA Increased serum levels of CCL11/eotaxin in schizophrenia. Prog. Neuropsychopharmacol. Biol. Psychiatry, 2007-11-23;32(3):710-4. 2007-11-23 [PMID: 18096286] (Human)
• Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Chauhan D, Munshi NC, Richardson PG, Anderson KC Targeting MEK1/2 blocks osteoclast differentiation, function and cytokine secretion in multiple myeloma. Br. J. Haematol., 2007-10-01;139(1):55-63. 2007-10-01 [PMID: 17854307] (Human)
• Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses. J. Biol. Chem., 2007-08-31;282(44):31882-90. 2007-08-31 [PMID: 17766241] (Human)
• Brito A, Almeida A, Gonsalez CR, Mendonca M, Ferreira F, Fernandes SS, Duarte AJ, Casseb J Successful HAART is associated with high B-chemokine levels in chronic HIV type 1-infected patients. AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):906-12. 2007-07-01 [PMID: 17678475] (Human)
• Birrell MA, Wong S, Catley MC, Belvisi MG Impact of tobacco-smoke on key signaling pathways in the innate immune response in lung macrophages. J. Cell. Physiol., 2008-01-01;214(1):27-37. 2008-01-01 [PMID: 17541958] (Human)
• Walker J, Tough DF Modification of TLR-induced activation of human dendritic cells by type I IFN: synergistic interaction with TLR4 but not TLR3 agonists. Eur. J. Immunol., 2006-07-01;36(7):1827-36. 2006-07-01 [PMID: 16783851] (Human)
• Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease. J. Pharmacol. Exp. Ther., 2006-05-11;318(2):741-50. 2006-05-11 [PMID: 16690722] (Human)
• Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission. Parasitology, 2005-03-01;130(0):333-42. 2005-03-01 [PMID: 15796016] (Human)
• Holl V, Hemmerter S, Burrer R, Schmidt S, Bohbot A, Aubertin AM, Moog C Involvement of Fc gamma RI (CD64) in the mechanism of HIV-1 inhibition by polyclonal IgG purified from infected patients in cultured monocyte-derived macrophages. J. Immunol., 2004-11-15;173(10):6274-83. 2004-11-15 [PMID: 15528366] (Human)
• Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue. Ann. Neurol., 2004-02-01;55(2):221-35. 2004-02-01 [PMID: 14755726] (Human)
• Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells. J. Leukoc. Biol., 2004-01-23;75(3):504-14. 2004-01-23 [PMID: 14742635] (Human)
• Townson JR, Barcellos LF, Nibbs RJ Gene copy number regulates the production of the human chemokine CCL3-L1. Eur. J. Immunol., 2002-10-01;32(10):3016-26. 2002-10-01 [PMID: 12355456] (Human)

Bioinformatics

• Gene Symbol: CCL3