Human C-Reactive Protein/CRP Quantikine ELISA Kit Summary
The Quantikine Human CRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CRP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CRP showed linear curves that were parallel to the standard curves obtained using the Quantikin...e kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CRP.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human C-Reactive Protein/CRP Quantikine ELISA Kit
C-reactive protein, pentraxin-related
C-Reactive Protein (CRP), also known as Pentraxin 1, is a non-glycosylated protein in the
Pentraxin family that also includes Pentraxin 2/SAP and Pentraxin 3/TSG-14. CRP functions as a
sensor and activator of the innate immune response (1). In humans, it is a major acute-phase
protein; its circulating concentration is dramatically elevated at the onset of inflammation (2).
In mice, however, serum CRP levels increase only slightly during inflammation, and the
analogous acute phase role is filled by Pentraxin 2 (3). CRP assembles non-covalently into a
110-120 kDa cyclical pentamer (4). Mature human CRP shares 71% and 64% amino acid (aa)
sequence identity with mouse and rat CRP, respectively (5).
CRP binds and opsonizes apoptotic cells (6-8) as well as bacteria such as S. pneumoniae (9, 10).
It subsequently enhances the phagocytosis of these opsonized cells (6, 8-10). CRP additionally
binds several proteins in the complement cascade including C1q, C4BP, and Factor H (8, 11-13).
It enhances activation of the classical complement pathway and the deposition of C3b (9). In
later stages of the response, CRP inhibits complement-mediated cell lysis through its binding
to C4BP and Factor H (8, 12). These interactions induce the upregulation of complement
inhibitory proteins CD46, CD59, and CD55/DAF and inhibit assembly of the membrane attack
complex (MAC) (8, 14).
CRP binds to Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells (15-17), and
Fc receptors are required for the phagocytosis of CRP-opsonized target cells (6, 10, 18). CRP
binding to Fc gamma RI induces Src activation which subsequently triggers the inhibitory Fc gamma RIIb and
dampens the inflammatory response (15, 19). CRP additionally promotes dendritic cell
maturation and humoral immunity (10). In cardiovascular disease, CRP binds to oxidized LDL,
exacerbates tissue damage in coronary artery infarction, and inhibits the repair of injured
vascular endothelium (7, 19, 20).