Human B7-H3 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human B7-H3 Quantikine ELISA Kit Summary

• Background: The Quantikine Human B7-H3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human B7-H3 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human B7-H3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human B7-H3 showed linear curves that were parallel to the standard curves obtained usin...g the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human B7-H3.
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• Specificity: Natural and recombinant human B7-H3
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CD276

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human B7-H3 Quantikine ELISA Kit

• B7H3
• B7-H3
• B7H34Ig-B7-H3
• B7-H3B7 homolog 3
• CD276 antigen
• CD276 molecule
• CD276
• Costimulatory molecule

Background

B7-H3 is an immunoglobulin superfamily protein that is expressed by osteoblasts, fibroblasts, ciliated respiratory epithelial cells, anterior pituitary progenitor cells, first trimester extravillous trophoblasts, and multiple tumor cells. It is induced on activated dendritic cells (DC), skeletal muscle myocytes, and monocytes. B7-H3 can both stimulate and inhibit immune cell activation. As a co-stimulator, B7-H3 augments macrophage activation and promotes proliferation and cytokine secretion by CD4+ and CD8+ T cells. As a co-inhibitor, tumor cell B7-H3 blocks the cytolytic activity of NK cells, limits cytokine production by activated T cells, and imparts an immunosuppressive phenotype to dendritic cells. B7-H3 can interact with TREML2/TLT-2 to enhance T cell activation including the CD8+ T cell-mediated lysis of tumor cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for B7-H3/CD276 (DB7H30)(5)

Publications using DB7H30

• Li, D;Wang, R;Liang, T;Ren, H;Park, C;Tai, CH;Ni, W;Zhou, J;Mackay, S;Edmondson, E;Khan, J;Croix, BS;Ho, M; Camel nanobody-based B7-H3 CAR-T cells show high efficacy against large solid tumours Nature communications 2023-09-22 [PMID: 37739951] (Mouse, Human)
• O Bostanci, P Sayin, R Kiziltan, S Algul, MA Aydin, O Kemik B7-H3: A Useful Emerging Diagnostic Marker for Colon Cancer BioMed Research International, 2022-12-27;2022(0):1523338. 2022-12-27 [PMID: 36605103] (Human)
• Taito Miyamoto, Ryusuke Murakami, Junzo Hamanishi, Kenji Tanigaki, Yuko Hosoe, Nathan Mise et al. B7-H3 Suppresses Antitumor Immunity via the CCL2–CCR2–M2 Macrophage Axis and Contributes to Ovarian Cancer Progression Cancer Immunology Research 2022-01-01 [PMID: 34799346] (Human)
• Y Liu, C Cheng, L Bai, F Yao, S Shi, Y Zhang Value of bile soluble B7H3 for the diagnosis of malignant biliary strictures: Results of a retrospective study Surg Oncol, 2019-01-29;28(0):195-200. 2019-01-29 [PMID: 30851900] (Human)
• Chao Xie Soluble B7-H3 promotes the invasion and metastasis of pancreatic carcinoma cells through the TLR4/NF-?B pathway Sci Rep, 2016-06-08;6(0):27528. 2016-06-08 [PMID: 27273624] (Human)

FAQs for B7-H3/CD276 (DB7H30). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CD276