Human HTRA1/PRSS11 ELISA Kit (Colorimetric)

ELISA: Human HTRA1/PRSS11 ELISA Kit (Colorimetric) [NBP2-76585] - Samples were spiked with high concentrations of Human HTRA1/PRSS11 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human HTRA1/PRSS11 ELISA Kit (Colorimetric) [NBP2-76585] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL

Human HTRA1/PRSS11 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human HTRA1 in samples. No significant cross-reactivity or interference between Human HTRA1 and analogues was observed.
• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.64
• Intra-Assay: CV% < 4.5
• Spike Recovery: 85-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HTRA1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human HTRA1/PRSS11 ELISA Kit (Colorimetric)

• ARMD7
• EC 3.4.21
• EC 3.4.21.108
• High-temperature requirement A serine peptidase 1
• HtrA serine peptidase 1
• HTRA1
• IGFBP5-protease
• L56
• ORF480
• PRSS11
• Serine protease 11
• serine, 11 (IGF binding)

Background

htrA1 encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HTRA1/PRSS11 ELISA Kit (NBP2-76585)(1)

Publication using NBP2-76585

• Chang YJ, Jenny LA, Li YS et al. CRISPR editing demonstrates rs10490924 raised oxidative stress in iPSC-derived retinal cells from patients with ARMS2/HTRA1-related AMD Proceedings of the National Academy of Sciences of the United States of America 2023-05-09 [PMID: 37126685] (ELISA)

FAQs for HTRA1/PRSS11 ELISA Kit (NBP2-76585). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HTRA1