HSPA8/HSC71/Hsc70 Antibody [Janelia Fluor® 669]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Janelia Fluor 669

HSPA8/HSC71/Hsc70 Antibody [Janelia Fluor® 669] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: E. coli-derived recombinant human HSPA8
  Asp534-Gly615
  Accession # P11142
• Specificity: Detects human, mouse and rat HSPA8/HSC71, Isoform 1 in Western blots. In Western blots, this antibody shows no cross‑reactivity with recombinant human HSPA1A/HSP70, HSPA2, HSPA6, GRP75 or GRP78.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HSPA8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product AF4148. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for HSPA8/HSC71/Hsc70 Antibody [Janelia Fluor® 669]

• Heat shock 70 kDa protein 8
• heat shock 70kD protein 8
• heat shock 70kDa protein 8
• heat shock cognate 71 kDa protein
• heat shock cognate protein 54
• heat shock cognate protein, 71-kDa
• HSC54
• HSC70
• HSC70heat shock 70kd protein 10
• HSC71
• HSP71
• HSP73
• HSP73constitutive heat shock protein 70
• HSPA10
• HSPA8
• LAP1
• lipopolysaccharide-associated protein 1
• LPS-associated protein 1
• MGC131511
• MGC29929
• NIP71
• N-myristoyltransferase inhibitor protein 71

Background

Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA8