Hsp90A/B Antibody (Hyb-K41220A)

Western Blot: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - analysis of Human Cell lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody (NB110-96870) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.

Immunohistochemistry: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-96870) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Immunocytochemistry/Immunofluorescence: Hsp90A/B Antibody (Hyb-K41220A) [NB110-96870] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody, Clone K41220A (NB110-96870). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 alpha/beta Monoclonal Antibody (NB110-96870) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 alpha/beta Antibody. (C) Composite.

• Reactivity: Hu, Mu, Rt, Ye
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: Hyb-K41220A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Hsp90A/B Antibody (Hyb-K41220A) Summary

• Immunogen: Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304
• Localization: Cytoplasm , Melanosome
• Specificity: Detects 90kD proteins corresponding to the molecular mass of hsp90 alpha or beta.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSP90AA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Hsp90A/B Antibody (Hyb-K41220A)

• FLJ31884
• Heat shock 86 kDa
• heat shock 90kD protein 1, alpha
• heat shock 90kD protein 1, alpha-like 4
• heat shock 90kD protein, alpha-like 4
• heat shock 90kDa protein 1, alpha
• heat shock protein 90kDa alpha (cytosolic), class A member 1
• heat shock protein HSP 90-alpha
• Hsp89
• Hsp90
• HSP90AHSP86
• HSP90N
• HSPC1HSP 86
• HSPCAHSP89A
• HSPCAL1
• HSPCAL4
• HSPN
• LAP2
• Renal carcinoma antigen NY-REN-38

Background

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms alpha and beta, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90alpha exists predominantly as a homodimer while HSP90beta exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSP90AA1
• Entrez:
  • 299331(Rat)
• Uniprot:
  • P08238()