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Hsp90A/B Antibody (Hyb-K41220A) [DyLight 350]

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Product Details

Summary
Reactivity Hu, Mu, Rt, YeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
Hyb-K41220A
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB110-96870AF405
Alexa Fluor 488
NB110-96870AF488
Alexa Fluor 647
NB110-96870AF647
Alexa Fluor 700
NB110-96870AF700
Biotin
NB110-96870B
DyLight 405
NB110-96870V
DyLight 488
NB110-96870G
DyLight 550
NB110-96870R
DyLight 650
NB110-96870C
DyLight 680
NB110-96870FR
DyLight 755
NB110-96870IR
FITC
NB110-96870F
HRP
NB110-96870H
Janelia Fluor 549
NB110-96870JF549
Janelia Fluor 646
NB110-96870JF646
Unconjugated
NB110-96870
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Hsp90A/B Antibody (Hyb-K41220A) [DyLight 350] Summary

Immunogen
Recombinant human HSP90alpha; Specificity mapped to amino acids 291-304
Localization
Cytoplasm, Melanosome
Specificity
Detects 90kD proteins corresponding to the molecular mass of hsp90 alpha or beta.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSP90AA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Hsp90A/B Antibody (Hyb-K41220A) [DyLight 350]

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • Hsp89
  • Hsp90
  • HSP90AHSP86
  • HSP90N
  • HSPC1HSP 86
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: HspA4 Antibody (SD0861) [NBP2-67817] - Staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HspA4 Antibody (SD0861) [NBP2-67817] - Analysis of paraffin-embedded rat brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
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Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
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Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.
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Immunohistochemistry-Paraffin: RRAS Antibody - Unpurified [NB100-56161] - RRAS was detected in immersion fixed paraffin-embedded sections of human placenta using this antibody at 1:500 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane in decidual and trophoblast cells.
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Knockdown Validated: Tmp21/p23 Antibody [NB110-57587] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57587) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reduc
Western Blot: TMP21 Antibody [NB110-57587] - Detection of TMP21 in human liver lysate using NB110-57587.
Immunohistochemistry-Paraffin: Tmp21/p23 Antibody [NB110-57587] - Tmp21/p23 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human TMP21/p23 polyclonal Antibody (Catalog # NB110-57587) at 1 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.
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gp96/HSP90B1/GRP94
Antibody (9G10)
NB300-619
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Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.
(2 Reviews)
(1 Publication)
Species: Hu, Mu, Rt, Bv, Ch, Ha
Applications: WB, ICC/IF, IHC, IHC-P, IP, ICC
Hsp90A/B Antibody
(Hyb-K41220A) [DyLigh ...
NB110-96870UV
Hsp90A/B Antibody (Hyb-K41220A) [DyLight 350]
Species: Hu, Mu, Rt, Ye
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB110-96870AF405
Alexa Fluor 488 NB110-96870AF488
Alexa Fluor 647 NB110-96870AF647
Alexa Fluor 700 NB110-96870AF700
Biotin NB110-96870B
DyLight 350 NB110-96870UV
DyLight 405 NB110-96870V
DyLight 488 NB110-96870G
DyLight 550 NB110-96870R
DyLight 650 NB110-96870C
DyLight 680 NB110-96870FR
DyLight 755 NB110-96870IR
FITC NB110-96870F
HRP NB110-96870H
Janelia Fluor 549 NB110-96870JF549
Janelia Fluor 646 NB110-96870JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol HSP90AA1