HSP90 Antibody (D7A)

Images

 
Western Blot: HSP90 Antibody (D7alpha) [NB110-96872] - analysis of Rat cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7Alpha. Load: 15 ug protein. Block: 1.5% BSA for ...read more
Immunohistochemistry-Paraffin: HSP90 Antibody (D7alpha) [NB110-96872] - analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: ...read more
Immunohistochemistry-Paraffin: HSP90 Antibody (D7alpha) [NB110-96872] - analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP, MiAr
Clone
D7A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSP90 Antibody (D7A) Summary

Immunogen
Full length protein HSP90 purified from chicken brain
Localization
Cytoplasm, Melanosome
Specificity
Recognizes 90kDa. Can isolate complexes of HSP90, Src kinase and cec37.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSP90AA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 5 ug
  • Microarray
Application Notes
2 ug/ml was sufficient for detection of HSP90a in 20 ug of heat shocked HeLa cell lysate as well as in 100 ng of human HSP90a protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.HSP90 Antibody.
Control
HSP90 Knockout 293T Cell Lysate
Publications
Read Publications using
NB110-96872 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Based on homology, It is predicted to detect Human, Mouse, Rat, Bovine, Porcine and Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.2 and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSP90 Antibody (D7A)

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • Hsp89
  • HSP90
  • HSP90AHSP86
  • HSP90N
  • HSPC1HSP 86
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Background

Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (4-7). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-96872
Species: Hu, Mu, Rt, Po, Bv, Ch, Rb
Applications: WB, ELISA, IHC, IHC-P, IP, MiAr

Publications for HSP90 Antibody (NB110-96872)(2)

We have publications tested in 1 confirmed species: Chicken.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Chicken
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HSP90 Antibody (NB110-96872) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB110-96872AF405
Alexa Fluor 488 NB110-96872AF488
Alexa Fluor 647 NB110-96872AF647
Alexa Fluor 700 NB110-96872AF700
Biotin NB110-96872B
DyLight 350 NB110-96872UV
DyLight 405 NB110-96872V
DyLight 488 NB110-96872G
DyLight 550 NB110-96872R
DyLight 650 NB110-96872C
DyLight 680 NB110-96872FR
DyLight 755 NB110-96872IR
FITC NB110-96872F
HRP NB110-96872H
Janelia Fluor 549 NB110-96872JF549
Janelia Fluor 646 NB110-96872JF646

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Blogs on HSP90.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

HSP90 - an essential eukaryotic protein with implications for drug development
The heat-shock protein 90 (HSP90) family is a group of highly conserved molecular chaperones with important functions in protein folding and in signal transduction. The HSP90 protein structure is so well conserved that some HSP90 antibodies are rea...  Read full blog post.

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ITGB1BP2 is a muscle-specific protein cloned by a rat created by Branccio's group in Italy that was found to interact with the cytoplasmic domain of integrin beta 11. It is expressed only in heart and skeletal muscle but is not essential for normal de...  Read full blog post.

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Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
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Bioinformatics

Gene Symbol HSP90AA1
Entrez
Uniprot
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