HSP90 alpha Antibody (5G5) - BSA Free

Western Blot: HSP90 alpha Antibody (5G5) [NBP2-22208] - Western blot analysis using Hsp90A mouse mAb against NIH3T3 (1), HeLa (2), HCT116(3), HL-60 (4) and C0S7 (5) cell lysate.

Immunohistochemistry-Paraffin: HSP90 alpha Antibody (5G5) [NBP2-22208] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Hsp90A mouse mAb with DAB staining.

Flow Cytometry: HSP90 alpha Antibody (5G5) [NBP2-22208] - Flow cytometric analysis of HeLa cells using Hsp90A mouse mAb (green) and negative control (red).

Western Blot: HSP90 alpha Antibody (5G5) [NBP2-22208] - Western blot analysis using Hsp90A mAb against human HSP90AA1 (AA: 275-484) recombinant protein. (Expected MW is 50.5 kDa)

Immunohistochemistry-Paraffin: HSP90 alpha Antibody (5G5) [NBP2-22208] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Hsp90A mouse mAb with DAB staining.

ELISA: HSP90 alpha Antibody (5G5) [NBP2-22208] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 5G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HSP90 alpha Antibody (5G5) - BSA Free Summary

• Description: Novus Biologicals Mouse HSP90 alpha Antibody (5G5) - BSA Free (NBP2-22208) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human HSP90 alpha expressed in E. Coli.
• Localization: Nucleus, Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSP90AA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HSP90 alpha Antibody (5G5) - BSA Free

• FLJ31884
• Heat shock 86 kDa
• heat shock 90kD protein 1, alpha
• heat shock 90kD protein 1, alpha-like 4
• heat shock 90kD protein, alpha-like 4
• heat shock 90kDa protein 1, alpha
• heat shock protein 90kDa alpha (cytosolic), class A member 1
• heat shock protein HSP 90-alpha
• HSP86
• Hsp89
• HSP89A
• HSP90 alpha
• Hsp90
• HSP90A
• HSP90AA1
• HSP90AHSP86
• HSP90N
• HSPC1
• HSPC1HSP 86
• HSPCA
• HSPCAHSP89A
• HSPCAL1
• HSPCAL4
• HSPN
• LAP2
• Renal carcinoma antigen NY-REN-38

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSP90AA1