HSP20/HSPB6 Antibody

Western Blot: HSP20/HSPB6 Antibody [NB120-13491] - Lane 1: MW Marker, Lane 2: HeLa (heat shocked), Lane 3: 3T3 (heat shocked), Lane 4: PC-12 (heat shocked).

• Reactivity: Hu, Mu, Rt, Bv, Ca, Fi
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

HSP20/HSPB6 Antibody Summary

• Description: Novus Biologicals Rabbit HSP20/HSPB6 Antibody (NB120-13491) is a polyclonal antibody validated for use in WB. Anti-HSP20/HSPB6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Rat skeletal muscle Hsp20.
• Specificity: Hsp20
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPB6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 (colorimetric)
• Application Notes: Detects a band of ~20 kDa by Western blot.
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Phosphate (pH 7.0) and 3% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HSP20/HSPB6 Antibody

• FLJ32389
• Heat shock 20 kDa-like protein p20
• heat shock protein beta-6
• heat shock protein, alpha-crystallin-related, B6
• HSP20
• HSPB6

Background

Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP20/HSPB6 Antibody (NB120-13491)(1)

Publication using NB120-13491

• Ju YT, Kwag SJ, Park HJ et al. Decreased expression of heat shock protein 20 in colorectal cancer and its implication in tumorigenesis. J. Cell. Biochem. 2014-09-04 [PMID: 25187324] (WB)

Bioinformatics

• Gene Symbol: HSPB6
• Entrez:
  • 126393(Human)
  • 243912(Mouse)
  • 192245(Rat)
• Uniprot:
  • P97541()