HSPB3 Overexpression Lysate

Images

 
Western Blot: HSPB3 Overexpression Lysate (Adult Normal) [NBP2-07644] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HSPB3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HSPB3 Overexpression Lysate Summary

Description

HSPB3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC220525

Accession#: NM_006308

Protein Tag: C-MYC/DDK
Gene
HSPB3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
16.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HSPB3 Overexpression Lysate

  • DHMN2C
  • heat shock 17 kDa protein
  • heat shock 27kDa protein 3
  • HMN2C
  • HSP 17
  • HSP27
  • HSPL27
  • protein 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-75477
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP2-67817
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
291-G1
Species: Hu
Applications: BA
DGB300
Species: Hu
Applications: ELISA
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP1-77685
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
210-TA
Species: Hu
Applications: BA
NBP1-77397
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
AF8691
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
H00003845-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IP, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
D6050
Species: Hu
Applications: ELISA
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-37568
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-00764
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
H00010598-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-81575
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-07644
Species: Hu
Applications: WB

Publications for HSPB3 Lysate (NBP2-07644) (0)

There are no publications for HSPB3 Lysate (NBP2-07644).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSPB3 Lysate (NBP2-07644) (0)

There are no reviews for HSPB3 Lysate (NBP2-07644). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HSPB3 Lysate (NBP2-07644) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HSPB3 Products

Array NBP2-07644

Bioinformatics Tool for HSPB3 Lysate (NBP2-07644)

Discover related pathways, diseases and genes to HSPB3 Lysate (NBP2-07644). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HSPB3 Lysate (NBP2-07644)

Discover more about diseases related to HSPB3 Lysate (NBP2-07644).
 

Pathways for HSPB3 Lysate (NBP2-07644)

View related products by pathway.

PTMs for HSPB3 Lysate (NBP2-07644)

Learn more about PTMs related to HSPB3 Lysate (NBP2-07644).

Blogs on HSPB3

There are no specific blogs for HSPB3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HSPB3 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol HSPB3