HSP10/EPF Antibody

Images

 
Western Blot: Hsp10 Antibody [NBP2-16890] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HSP10/EPF Antibody [NBP2-16890] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 15 min. Green: HSP10 protein stained by HSP10 antibody diluted ...read more
Immunohistochemistry-Paraffin: HSP10/EPF Antibody [NBP2-16890] - Paraffin-embedded lung carcinoma. HSP10 antibody dilution: 1:250.
Immunocytochemistry/ Immunofluorescence: HSP10/EPF Antibody [NBP2-16890] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP10 protein stained by HSP10 antibody diluted at 1:100. Red: alpha ...read more
Immunocytochemistry/ Immunofluorescence: HSP10/EPF Antibody [NBP2-16890] - U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP10 protein stained by HSP10 antibody diluted at 1:200. Red: alpha ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HSP10/EPF Antibody Summary

Immunogen
Full length human Cpn10 Recombinant protein.
Localization
Mitochondrion matrix
Predicted Species
Rat (98%), Porcine (100%), Bovine (100%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (80%), Japanese Medaka (83%), Xenopus laevis (88%),Chicken (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP10/EPF Antibody

  • 10 kDa chaperonin
  • 10 kDa heat shock protein, mitochondrial
  • Chaperonin 10 Homolog
  • Chaperonin 10
  • CPN10HSP10
  • Early-pregnancy factor
  • EPF
  • GroES
  • heat shock 10kD protein 1 (chaperonin 10)
  • heat shock 10kDa protein 1 (chaperonin 10)
  • HSP10
  • HSPE1

Background

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSPE1
Entrez
Uniprot