HSP10/EPF Antibody - BSA Free

Western Blot: HSP10/EPF Antibody [NB110-11473] - Western blot analysis of Rat brain cell lysates showing detection of HSP10/EPF protein using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - Immunohistochemistry analysis using Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP10/EPF Polyclonal Antibody (NB110-11473) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..

Western Blot: HSP10/EPF Antibody [NB110-11473] - analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - analysis using Rabbit Anti-Cpn10 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Gp, Rb, Sh, Xp
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HSP10/EPF Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HSP10/EPF Antibody - BSA Free (NB110-11473) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human Cpn10 peptide AA 91-101
• Localization: Mitochondrial
• Specificity: Rabbit Polyclonal to Cpn10
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Control:

  HSP10/EPF Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HSP10/EPF Antibody - BSA Free

• 10 kDa chaperonin
• 10 kDa heat shock protein, mitochondrial
• Chaperonin 10 Homolog
• Chaperonin 10
• CPN10HSP10
• Early-pregnancy factor
• EPF
• GroES
• heat shock 10kD protein 1 (chaperonin 10)
• heat shock 10kDa protein 1 (chaperonin 10)
• HSP10
• HSPE1

Background

Homologs of E. coli Cpn10 make up a family of small heat shock proteins with an approximate molecular mass of 10 kDa (Hsp10s). Cpn10 acts as a co-chaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, Cpn10 is thought to interact with cHsp60, a reported immunopathological antigen, identifying Cpn10 as a potential contributor to an immunological response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for HSP10/EPF Antibody (NB110-11473). (Showing 1 - 1 of 1 FAQ).

1. I am wondering if the HSP10 with catalog number NB110-11473 is a primary (capture antibody) suitable for human sample? Also do you have pairs antibody for HSP60, HSP70?
   • With regards to your question, NB110-11473 is a rabbit polyclonal antibody which also cross reacts with human. We do have the HSP60 and HSP70 antibodies for ELISA as well. If you are interested in ordering these antibodies, please find more detailed information in the following links. H00003329-AP21 and H00003308-PW1.

Bioinformatics

• Gene Symbol: HSPE1
• Entrez:
  • 3336(Human)
  • 15528(Mouse)
  • 25462(Rat)
• Uniprot:
  • P61604()