HSD3B7 Overexpression Lysate (Native)

Images

 
Western Blot: HSD3B7 Overexpression Lysate (Adult Normal) [NBL1-11734] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HSD3B7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HSD3B7 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202468 and based on accession number NM_025193. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, mRNA.
Gene
HSD3B7

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
40.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HSD3B7

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HSD3B7 Overexpression Lysate (Native)

  • 3 beta-hydroxy-delta 5-C27-steroid oxidoreductase
  • 3 beta-hydroxysteroid dehydrogenase type 7
  • 3 beta-hydroxysteroid dehydrogenase type VII
  • 3-beta-HSD VII
  • 7-alpha-diol 3-beta-dehydrogenase
  • C(27) 3-beta-HSD
  • C(27)-3BETA-HSD
  • Cholest-5-ene-3-beta
  • EC 1.1.1.-
  • EC 1.1.1.181
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7,3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase
  • PFIC4
  • SDR11E3
  • short chain dehydrogenase/reductase family 11E, member 3

Background

This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol HSD3B7