HSD3B7 Antibody - BSA Free

Immunohistochemistry: HSD3B7 Antibody - BSA Free [NBP3-46073] - Immunohistochemistry of paraffin-embedded human pancreas cancer using (HSD3B7 antibody) at dilution of 1:50

Western Blot: HSD3B7 Antibody - BSA Free [NBP3-46073] - Mouse liver tissue were subjected to SDS PAGE followed by western blot with (HSD3B7 antibody) at dilution of 1:500

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSD3B7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HSD3B7 Antibody - BSA Free (NBP3-46073) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 7. (Uniprot# Q9H2F3)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSD3B7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HSD3B7 Antibody - BSA Free

• 3 beta-hydroxy-delta 5-C27-steroid oxidoreductase
• 3 beta-hydroxysteroid dehydrogenase type 7
• 3 beta-hydroxysteroid dehydrogenase type VII
• 3-beta-HSD VII
• 7-alpha-diol 3-beta-dehydrogenase
• C(27) 3-beta-HSD
• C(27)-3BETA-HSD
• Cholest-5-ene-3-beta
• EC 1.1.1.-
• EC 1.1.1.181
• hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7,3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase
• PFIC4
• SDR11E3
• short chain dehydrogenase/reductase family 11E, member 3

Background

HSD3B7 codes for a protein with a length of 369 amino acids and a weight of approximately 41 kDa, with a shorter isoform with a length of 196 amino acids and a weight of approximately 21 kDa, and is a protein that plays a role in the biosynthesis of all classes of hormonal steroids, as it does not metabolize several different C steroids as substrates. It is also involved in bile acid synthesis from cholesterol. Current studies are being done on diseases and disorders relating to this gene including congenital bile acid synthesis defect, hepatitis, jaundice, cholesterol, tuberculosis, and neuronitis. HSD3B7 has also been shown to have interactions with PLSCR1, AKR1D1, CYP27A1, CYP39A1, and CYP7A1 in pathways such as the bile acid biosynthesis and metabolic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSD3B7