HSD17B13 Antibody - Azide and BSA Free

Western Blot: HSD17B13 Antibody [NBP3-04837] - Analysis of extracts of various cell lines, using HSD17B13 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: HSD17B13 Antibody [NBP3-04837] -Human liver cancer using HSD17B13 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry-Paraffin: HSD17B13 Antibody [NBP3-04837] - Human liver using HSD17B13 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: HSD17B13 Antibody - Azide and BSA Free [HSD17B13] - Immunofluorescence analysis of paraffin-embedded human liver using HSD17B13 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: HSD17B13 Antibody - Azide and BSA Free [HSD17B13] - Immunofluorescence analysis of paraffin-embedded rat liver using HSD17B13 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: HSD17B13 Antibody - Azide and BSA Free [HSD17B13] - Immunofluorescence analysis of paraffin-embedded mouse liver using HSD17B13 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HSD17B13 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit HSD17B13 Antibody - Azide and BSA Free (NBP3-04837) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-211 of human HSD17B13 (NP_835236.2). RRKSVAGEIVLITGAGHGIGRQTTYEFAKRQSILVLWDINKRGVEETAAECRKLGVTAHAYVVDCSNREEIYRSLNQVKKEVGDVTIVVNNAGTVYPADLLSTKDEEITKTFEVNILGHFWITKALLPSMMERNHGHIVTVASVCGHEGIPYLIPYCSSKFAAVGFHRGLTSELQALGKTGI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSD17B13
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for HSD17B13 Antibody - Azide and BSA Free

• HMFN0376
• hydroxysteroid (17-beta) dehydrogenase 13
• member 3,17-beta-hydroxysteroid dehydrogenase 13
• MGC138508,17-beta-HSD 13
• NIIL497
• SCDR9
• short-chain dehydrogenase/reductase 9
• UNQ497/PRO1014

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSD17B13