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15-Lipoxygenase 2 Antibody

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Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of extracts of various cell lines, using ALOX15B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 2s.
Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of extracts of various cell lines, using ALOX15B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of U2OS cells using ALOX15B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of NIH/3T3 cells using ALOX15B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of PC-12 cells using ALOX15B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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15-Lipoxygenase 2 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALOX15B (NP_001132.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALOX15B
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
Publications
Read Publication using
NBP2-92668 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for 15-Lipoxygenase 2 Antibody

  • 15-lipoxygenase 2
  • 15-LOX-B
  • arachidonate 15-lipoxygenase 2
  • arachidonate 15-lipoxygenase B
  • Arachidonate 15-lipoxygenase type II
  • arachidonate 15-lipoxygenase, second type
  • arachidonate 15-lipoxygenase, type B
  • arachidonate omega(6) lipoxygenase
  • EC 1.13.11
  • EC 1.13.11.33,15-LOX-215S-lipoxygenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)
Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 Lipoxygenase 1.
Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
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Immunohistochemistry-Paraffin: 12-Lipoxygenase Antibody [NBP1-90338] - Staining of human heart muscle shows distinct positivity in myocytes.
Western Blot: 12-Lipoxygenase Antibody [NBP1-90338] - Analysis in human cell line HELA.
Immunohistochemistry-Paraffin: 12-Lipoxygenase Antibody [NBP1-90338] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
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Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2527] - Staining in human fibroblasts. Image from verified customer review.
Immunohistochemistry: LOX Antibody [NB100-2527] - IR-enhanced LOX in tumor xenografts. Tumor tissue from A549-derived tumor xenografts treated with 1 x 10 Gy and 2 x 10 Gy (with 12 h between fractions) and evaluated by immunohistochemistry for LOX at different time points after irradiation. Whole tumor sections were quantified for specific LOX-staining intensity. Each treatment group consists of 3 animals. At least 3 sections per tumor/animal were analyzed. Image collected and cropped by CiteAb from the following publication (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY licence.
Western Blot: LOX Antibody [NB100-2527] - LOX secretion and activity in response to tumor cell irradiation. Western blot of LOX zymogen (50 kDa) and active form (32 kDa) in conditioned media derived from irradiated A549 lung carcinoma cells 16-20 h after irradiation and quantification of band intensities from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY licence.
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Knockdown Validated: 5-Lipoxygenase Antibody [NB110-58748] - Knockdown of 5-Lipoxygenase inhibits TNF-alpha-induced IL-6 and MCP-1 release in human synovial fibroblasts.Total protein extracts from RASFs transfected with empty vector or 5-Lipoxygenase shRNA were used for Western blotting. Immunoblotting showed that cells transfected with 5-Lipoxygenase shRNA of clone No.3 and No.7 markedly decreased 5-Lipoxygenase expression. Human synovial fibroblasts transfected with empty vector or 5-Lipoxygenase shRNA were treated with TNF-alpha for 6 hr. IL-6 and MCP-1 protein release in the conditioned medium was evaluated by ELISA. Note that knockdown of 5-Lipoxygenase decreased IL-6. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168259/) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody - BSA Free [NB110-58748] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with 5-Lipoxygenase Antibody (NB110-58748) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Simple Western: 5-Lipoxygenase Antibody [NB110-58748] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
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Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
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Immunohistochemistry: ALOX12B Antibody [NBP1-89409] - Analysis of ALOX12B and PTGIS proteins expression in tumor and non-tumor tissue by Immunohistochemistry. Expression of ALOX12B protein; the arrows show weak labeling (brown staining) of non-tumor squamous cells in (a) and OSCC cells in (b). Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/jo/2018/6301980/), licensed under a CC-BY licence.
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Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of extracts of various cell lines, using ALOX15B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 2s.
Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of NIH/3T3 cells using ALOX15B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Knockdown Validated: 5-Lipoxygenase Antibody [NB110-58748] - Knockdown of 5-Lipoxygenase inhibits TNF-alpha-induced IL-6 and MCP-1 release in human synovial fibroblasts.Total protein extracts from RASFs transfected with empty vector or 5-Lipoxygenase shRNA were used for Western blotting. Immunoblotting showed that cells transfected with 5-Lipoxygenase shRNA of clone No.3 and No.7 markedly decreased 5-Lipoxygenase expression. Human synovial fibroblasts transfected with empty vector or 5-Lipoxygenase shRNA were treated with TNF-alpha for 6 hr. IL-6 and MCP-1 protein release in the conditioned medium was evaluated by ELISA. Note that knockdown of 5-Lipoxygenase decreased IL-6. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168259/) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody - BSA Free [NB110-58748] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with 5-Lipoxygenase Antibody (NB110-58748) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Simple Western: 5-Lipoxygenase Antibody [NB110-58748] - Simple Western lane view shows a specific band for 5-Lipoxygenase in 0.5 mg/ml of Human Lung lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
(2 Reviews)
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Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Western Blot: COX5A Antibody [NBP1-32550] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: COX5A Antibody [NBP1-32550] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: COX5A Antibody [NBP1-32550] - Sample(30 ug whole cell lysate)A:HeLa S3 B:Hep G2 15% SDS PAGE, antibody diluted at 1:5000.
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Immunocytochemistry/Immunofluorescence: CPOX Antibody (36B10) [NBP2-59438] - Analysis of CPOX in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CPOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: CPOX Antibody (36B10) [NBP2-59438] - Lane 1: Recombinant protein CPOX, Lane 2: 293T cell lysates, Lane 3: HepG2 cell lysates, Lane 4: A549 cell lysates, Lane 5: Jurkat cell lysates, Lane 6: K562 cell lysates, Lane 7: LnCaP cell lysates
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COX8A GST (N-Term) Pr ...
H00001351-P01
SDS-Page: COX8A Recombinant Protein [H00001351-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
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IL-4 [Unconjugated]
6507-IL/CF
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL.
(3 Publications)
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Applications: BA
15-Lipoxygenase 2
Antibody
NBP2-92668
15-Lipoxygenase 2 Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF

Publications for 15-Lipoxygenase 2 Antibody (NBP2-92668)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol ALOX15B