HSD11B2 Antibody (921215) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 921215
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

HSD11B2 Antibody (921215) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human 11β‑HSD2
  Met105-Arg405
  Accession # P80365
• Specificity: Detects human 11 beta ‑HSD2 in direct ELISA and Western Blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSD11B2 Antibody (921215) [Alexa Fluor® 594]

• 11 betaHSD2
• 11 beta-HSD2
• AME
• corticosteroid 11-beta-dehydrogenase isozyme 2,11-beta-HSD2
• EC 1.1.1
• EC 1.1.1.-
• HSD11B2
• HSD11KAME1
• HSD2,11-DH2
• hydroxysteroid (11-beta) dehydrogenase 2
• NAD-dependent 11-beta-hydroxysteroid dehydrogenase
• SDR9C3
• short chain dehydrogenase/reductase family 9C member 3,11-beta-hydroxysteroid dehydrogenase type 2

Background

The human enzyme HSD-2/11 beta-HSD2 is encoded by the HSD11B2 gene and codes for a 405 aa protein, with high expression in kidney, colon, pancreas and placenta1. HSD-2 modulates intracellular glucocorticoid levels by catalyzing the conversion of cortisol to the inactive metabolite cortisone2. HSD-2 is abundantly expressed in human placenta and controls fetal glucocorticoid levels to protect the fetus from glucocorticoid exposure. Similarly, HSD-2 expression in the distal nephron protects mineralcorticoid receptors from glucocorticoids and helps regulate blood pressure3. Exposure to major environmental pollutant like Cadmium, inhibits activity of HSD-2 which affects the synthesis of glucocorticoids in placenta and thus contribute to developing preeclamptic conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.