Recombinant Human HR6A/UBE2A Protein

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SDS-Page: HR6A/UBE2A Protein [NBP1-44378] - UBE2A, 19.4 kDa (172aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human HR6A/UBE2A Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-152 of Human HR6A/UBE2A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
UBE2A
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human HR6A/UBE2A Protein

  • EC 6.3.2.19
  • HHR6A
  • HR6A
  • RAD6A
  • RAD6ARAD6 homolog A
  • UBC2
  • UBE2A
  • Ubiquitin carrier protein A
  • ubiquitin-conjugating enzyme E2 A
  • ubiquitin-conjugating enzyme E2A (RAD6 homolog)
  • Ubiquitin-protein ligase A

Background

Ubiquitin-conjugating enzyme E2 A isoform 1, also known as UBE2A is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. Recombinant human UBE2A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol UBE2A
Entrez