Recombinant Human HR6A/UBE2A Protein

Product Discontinued

Recombinant Human HR6A/UBE2A Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-152 of Human HR6A/UBE2A
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2A
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 19.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 1 mM EDTA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human HR6A/UBE2A Protein

• EC 6.3.2.19
• HHR6A
• HR6A
• RAD6A
• RAD6ARAD6 homolog A
• UBC2
• UBE2A
• Ubiquitin carrier protein A
• ubiquitin-conjugating enzyme E2 A
• ubiquitin-conjugating enzyme E2A (RAD6 homolog)
• Ubiquitin-protein ligase A

Background

Ubiquitin-conjugating enzyme E2 A isoform 1, also known as UBE2A is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. Recombinant human UBE2A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2A