![Western Blot: HPRT Antibody (4C3-G8) [H00003251-M01] - HPRT1 monoclonal antibody (M01), clone 4C3-G8 Analysis of HPRT1 expression in MCF-7.](http://images.novusbio.com/fullsize/HPRT-Antibody-4C3-G8-Western-Blot-H00003251-M01-img0002.jpg "Western Blot: HPRT Antibody (4C3-G8) [H00003251-M01] - HPRT1 monoclonal antibody (M01), clone 4C3-G8 Analysis of HPRT1 expression in MCF-7.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 4C3-G8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | HPRT1 (AAH00578, 1 a.a. ~ 218 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA |
| Specificity | HPRT1 - hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HPRT1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00003251-M01 | Applications | Species |
|---|---|---|
| Hayashi H, Naoi S, Nakagawa T et al. Sorting Nexin 27 Interacts with Multidrug Resistance-associated Protein 4 (MRP4) and Mediates Internalization of MRP4. J Biol Chem. 2012-03-12 [PMID: 22411990] |
Secondary Antibodies |
Isotype Controls |
Research Areas for HPRT Antibody (H00003251-M01)Find related products by research area.
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![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](https://images.novusbio.com/images/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")