HPRT Antibody (2G8)

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Western Blot: HPRT Antibody (AT2G8) [NBP1-74045] The extracts of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:500~1:5000). Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HPRT Antibody (2G8) Summary

Immunogen
Recombinant human HPRT (1-218aa) purified from E. coli
Specificity
Anti-human HPRT mAb, clone 2G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HPRT protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HPRT1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:100-1:2000
Application Notes
HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HPRT Antibody (2G8)

  • EC 2.4.2.8
  • HGPRTHPRTHGPRTase
  • hypoxanthine phosphoribosyltransferase 1
  • hypoxanthine-guanine phosphoribosyltransferase

Background

HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HPRT1
Uniprot