HP1 alpha Antibody - BSA Free

Western Blot: HP1 alpha Antibody [NBP2-59224] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1 alpha, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1 alpha, HP1 beta and HP1 gamma is indicated on the right. The Western blot analysis clearly demonstrates that the HP1 alpha antibody specifically recognizes HP1 alpha and not HP1 beta and HP1 gamma.

Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody [NBP2-59224] - HeLa cells were stained with the antibody against HP1alpha and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1alpha antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

• Reactivity: Hu
• Applications: WB, ICC/IF, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HP1 alpha Antibody - BSA Free Summary

• Immunogen: The exact immunogen is propietary information.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CBX5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunofluorescence 1:500
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Purity: Protein G purified

Alternate Names for HP1 alpha Antibody - BSA Free

• Antigen p25
• chromobox homolog 5 (Drosophila HP1 alpha)
• chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• chromobox homolog 5
• chromobox protein homolog 5
• Heterochromatin protein 1 homolog alpha
• heterochromatin protein 1-alpha
• HP1 alpha homolog
• HP1 alpha
• HP1
• HP1A
• HP1-ALPHA
• HP1Hs alpha
• HP1Hs-alpha

Background

Heterochromatin protein-1 (HP1) is a methyl-lysine binding adapter protein that is involved in a variety important functions in the cell nucleus. Localized at heterochromatin sites, HP1 is responsible for gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.

HP1 antibodies are useful tools for research on transcription regulation and epigenetic signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBX5