HOXB13 Antibody - BSA Free

Western Blot: HOXB13 Antibody [NBP2-43655] - Immunoblot of VCaP cells overexpressing the indicated SPOP (ubiquitin ligase adaptor) species. Protein expression changes were normalized to B-ACTIN and Control cell line, (n = 2). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33531470) licensed under a CC-BY license.

Western Blot: HOXB13 Antibody [NBP2-43655] - Analysis of A. 30 ug PC-3 whole cell extract B. 30 ug PC-3 nuclear extract 12 % SDS-PAGE HOXB13 antibody dilution: 1:1000.

Western Blot: HOXB13 Antibody [NBP2-43655] - Genetic alterations in SPOP & ERG are synthetic sick.a Distribution of genetic alterations in SPOP & ERG across 333 primary prostate cancers in TCGA database3,8,9. b 3D growth of mouse prostate epithelial organoids derived from C57BL/6 mice over-expressing the indicated SPOP & ERG species (bar represents 20 µm) (n = 3, technical replicates). Representative bright-field pictures & hematoxylin & eosin (H&E) stained sections are shown. c In vivo growth of VCaP xenografts over-expressing the indicated SPOP species in immune-compromised mice (each group, n = 10). d Immunoblot of VCaP cells overexpressing the indicated SPOP species & corresponding quantification of the indicated protein levels depicted as a heatmap. Protein expression changes were normalized to beta -ACTIN & Control cell line, (n = 2). e Tumor growth kinetics of xenografts established from LuCaP-147 PDX (SPOP-Y83C) stably overexpressing delta ERG or Control vector (each group, n = 10). Corresponding immunoblot & quantification are depicted as a heatmap. Protein expression changes were normalized to Vinculin (VCL) & Control cell line. f Dose–response curve of VCaP cells overexpressing the indicated SPOP species & treated with the ETS-inhibitor YK-4-279. All error bars, mean + s.e.m. P-values were determined by one-way ANOVA (b) or two-way ANOVA (c, e, f) with multiple comparisons & adjusted using Benjamini–Hochberg post-test ****P < 0.0001. Molecular weights are indicated in kilodaltons (kDa). Source data are provided as a Source Data File. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33531470), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HOXB13 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HOXB13 Antibody - BSA Free (NBP2-43655) is a polyclonal antibody validated for use in WB, IP and ChIP. Anti-HOXB13 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HOXB13. The exact sequence is proprietary.
• Predicted Species: Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HOXB13
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for HOXB13 Antibody - BSA Free

• homeo box B13
• homeobox B13
• homeobox protein Hox-B13
• HOXB13
• PSGD

Background

HOXB13 is a novel member of the AbdB subfamily of vertebrate HOX genes which are clustered in 4 unlinked complexes in the genome (HOXA, HOXB, HOXC, and HOXD clusters) which each span about 200 kb and contains 9 to 11 genes, transcribed from the same strand of DNA. The order of the HOX genes along the chromosome correlates with their expression along the anterior/posterior axis of the embryo and suggests that their organization is integral to their proper regulation. Members of the Abdominal B (AbdB) subfamily of homeobox genes exhibit posterior domains of expression, including the developing urogenital system, in vertebrates. Several HOXB genes have been described in humans and other mammals though it was thought that HOXB genes 10 through 13 had been lost in evolution until HOXB13 was discovered. HOXB13 gene contains 2 exons and encodes a 284-amino acid protein, 2 residues shorter than the mouse protein. It is separated from HOXB9 by 70 kb but is transcribed in the same orientation as the other HOXB genes. The 60-amino acid homeodomain, located near the C terminus of the protein, demonstrated 78 to 83% identity with HOX proteins in paralog group 13 of the AbdB subfamily; 6 of the amino acid differences from other member of this group represented conservative changes. HOXB13 had less than 60% identity to other AbdB-related genes. Mouse Hoxb13 is expressed first in the tailbud of embryonic mice and subsequently in the hindgut, urogenital tract, and the posterior extent of the spinal cord. It is not expressed in the secondary axes. During the first 2 trimesters of development, wound healing occurs without scars. Two homeobox genes, PRX2 and HOXB13, are differentially expressed during fetal versus adult wound healing. Both genes are expressed in proliferating fibroblasts and fetal dermis, but not in adult dermis. The HOXB13 gene has been mapped to human chromosome 17q21.2, where other HOXB genes are clustered, whilst the mouse Hoxb13 gene mapped to chromosome 11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOXB13 Antibody (NBP2-43655)(1)

Publication using NBP2-43655

• Bernasocchi, T, El Tekle, G Et al. Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer. Nat Commun 2021-02-02 [PMID: 33531470] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: HOXB13