Host Cell Factor 1/HCFC1 Antibody

Images

 
Western Blot: HCFC1 Antibody [NBP2-16786] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: HCFC1 Antibody [NBP2-16786] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HCFC1 Antibody [NBP2-16786] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:100 dilution.
Western Blot: HCFC1 Antibody [NBP2-16786] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:500.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.76 mg/ml

Order Details

Host Cell Factor 1/HCFC1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HCF1. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HCFC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.76 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Host Cell Factor 1/HCFC1 Antibody

  • C1 Factor
  • CFF
  • CFFC1 factor
  • HCF
  • HCF1
  • HCF1HFC1
  • HCF-1VP16 accessory protein
  • HCFC1
  • HFC1
  • host cell factor C1 (VP16-accessory protein)
  • MGC70925
  • VCAFhost cell factor 1

Background

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for Host Cell Factor 1/HCFC1 Antibody (NBP2-16786)

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Bioinformatics

Gene Symbol HCFC1
Entrez
Uniprot