HOOK1 Antibody - BSA Free

Immunoprecipitation:HOOK1 AntibodyNBP3-30164] -Detection of human HOOK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HOOK1 antibody NBP3-30164 (lot NBP3-30164-1) used for IP at 6 µg per reaction. HOOK1 was also immunoprecipitated by rabbit anti-HOOK1.. For blotting immunoprecipitated HOOK1, NBP3-30164 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HOOK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HOOK1 Antibody - BSA Free (NBP3-30164) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 150 and 200
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HOOK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for HOOK1 Antibody - BSA Free

• hHK1
• HK1h-hook1
• hook homolog 1 (Drosophila)
• MGC10642
• protein Hook homolog 1

Background

HOOK1 encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HOOK1