HO-2/HMOX2 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-2/HMOX2 at approximately 38 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HO‑2/HMOX2 at approximately 43 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HO‑2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western lane view shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HO‑2/HMOX2 at approximately 43 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HO‑2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HO-2/HMOX2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HO-2/HMOX2
  Met1-Met316
  Accession # P30519
• Specificity: The antibody is known to react with endogenous human, mouse, and rat HO-2 in in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HMOX2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HO-2/HMOX2 Antibody [Unconjugated]

• heme oxygenase (decycling) 2
• heme oxygenase 2
• HMOX2
• HO2
• HO-2
• HO-2EC 1.14.99.3

Background

Heme Oxygenase 2 (HO-2), also known as HMOX2, is a 36 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, the constitutively expressed heme oxygenase-2 (HO-2/HMOX2) and the inducible heme oxygenase-1 (HO-1/HMOX1). HO-1 expression is induced by heme and other non-heme compounds. Human HO-2 shares 42% amino acid sequence identity with human HO-1 and 89% amino acid sequence identity with mouse and rat HO-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMOX2
• Uniprot:
  • P30519()