HNRNPA0 Antibody - BSA Free

Western Blot: HNRNPA0 Antibody [NBP2-55847] - Western blot analysis in human cell line RT-4.

Immunocytochemistry/Immunofluorescence: HNRNPA0 Antibody [NBP2-55847] - Staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human cerebral cortex, endometrium, liver and lower gastrointestinal using Anti-HNRNPA0 antibody NBP2-55847 (A) shows similar protein distribution across tissues to independent antibody NBP1-83240 (B).

Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human colon shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: HNRNPA0 Antibody [NBP2-55847] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HNRNPA0 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HNRNPA0 Antibody - BSA Free (NBP2-55847) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: KLFVGGLKGDVAEGDLIEHFSQFGTVE
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPA0
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  HNRNPA0 Recombinant Protein Antigen (NBP2-55847PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HNRNPA0 Antibody - BSA Free

• heterogeneous nuclear ribonucleoprotein A0
• hnRNA binding protein
• hnRNPA0
• HNRPA0hnRNP A0

Background

HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPA0