hnRNP-Q Antibody

Western Blot: hnRNP-Q Antibody [NBP2-34068] - Analysis in human cell line Daudi.

Immunocytochemistry/Immunofluorescence: hnRNP-Q Antibody [NBP2-34068] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody [NBP2-34068] - Staining in human thyroid gland and pancreas tissues using anti-SYNCRIP antibody. Corresponding SYNCRIP RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody [NBP2-34068] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody [NBP2-34068] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: hnRNP-Q Antibody [NBP2-34068] - Staining of human thyroid gland shows high expression.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

hnRNP-Q Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: GNGTEEPMDTTSAVIHSENFQTLLDAG
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYNCRIP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  hnRNP-Q Protein (NBP2-34068PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for hnRNP-Q Antibody

• dJ3J17.2
• Glycine- and tyrosine-rich RNA-binding protein
• GRYRBP
• GRY-RBPNS1-associated protein 1
• heterogeneous nuclear ribonucleoprotein Q
• hnRNP Q
• hnRNP-Q
• HNRPQ
• HNRPQ1
• NSAP1FLJ31626
• PP68
• synaptotagmin binding, cytoplasmic RNA interacting protein
• Synaptotagmin-binding, cytoplasmic RNA-interacting protein

Background

Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYNCRIP
• Uniprot:
  • O60506(Human)